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Biophysical characterization of heme binding to the intrinsically disordered region of Bach1

  • Kei Segawa
  • Miki Watanabe-Matsui
  • Kengo Tsuda
  • Toshitaka Matsui
  • Mikako Shirouzu
  • Kazuhiko Igarashi
  • Kazutaka MurayamaEmail author
Original Article

Abstract

Transcriptional repressor Bach1 plays an important role in antioxidant response. Bach1 function is regulated by heme binding to the four cysteine-proline (CP) motifs in Bach1, which leads to inhibition of its activity. Three of these CP motifs are located N-terminal to the bZip (basic leucine zipper) domain that is responsible for DNA binding. Based on sequence analysis, the region surrounding these CP motifs was expected to be intrinsically disordered. Bach1 is one of few known intrinsically disordered proteins that accept multiple heme molecules for functional regulation, but the molecular mechanisms of heme binding and functional regulation remain unclear. Uncovering these mechanisms is important for understanding Bach1-mediated antioxidant response. Biophysical characterization revealed that 5-coordinated heme binding was unique to the CP motifs within the heme-binding region of Bach1, whereas 6-coordinated binding occurred nonspecifically. Comparison of the wild-type protein and a CP motif mutant indicated that the level of 6-coordinated heme binding was reduced in the absence of 5-coordinated heme binding. Analytical ultracentrifugation showed that the CP motif mutant protein had a more elongated conformation than the wild-type protein, suggesting that cysteines within the CP motifs contribute to intramolecular interactions in Bach1. Thus, heme binding at the CP motifs induces a global conformational change in the Bach1 heme-binding region, and this conformational change, in turn, regulates the biological activity of Bach1.

Keywords

Intrinsically disordered proteins Heme CP motif Protein conformation Cysteine reactivity 

Abbreviations

IPTG

Isopropyl β-d-1-thiogalactopyranoside

DTT

Dithiothreitol

TCEP

Tris(2-carboxyethyl)phosphine

MALDI-TOF MS

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

NMR

Nuclear magnetic resonance

NOESY

Nuclear Overhauser effect spectroscopy

TOCSY

Total correlation spectroscopy

Notes

Acknowledgements

We acknowledge the support of the Biomedical Research Core of the Tohoku University Graduate School of Medicine. This work was supported by a JSPS Grant-in-Aid for Scientific Research Number 18H04021 and 15H02506 (KI) and Research Fellowships for Young Scientists (Grant No. 16J40189) to M. W.-M. We thank Natasha Beeton-Kempen, Ph.D., from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.

Compliance with ethical standards

Conflict of interest

KI and KM received partial financial support from Teijin Pharma Co., Ltd. through a collaborative research contract.

Supplementary material

249_2019_1364_MOESM1_ESM.pptx (996 kb)
Supplementary file 1 (PPTX 995 kb)

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Copyright information

© European Biophysical Societies' Association 2019

Authors and Affiliations

  1. 1.Division of Biomedical Measurements and Diagnostics, Graduate School of Biomedical EngineeringTohoku UniversityAobaJapan
  2. 2.Pharmaceutical Discovery Research LaboratoriesTeijin Pharma LimitedTokyoJapan
  3. 3.Department of BiochemistryTohoku University Graduate School of MedicineSendaiJapan
  4. 4.Japan Society for the Promotion of Science (JSPS)TokyoJapan
  5. 5.Laboratory for Protein Functional and Structural BiologyRIKEN Center for Biosystems Dynamics ResearchYokohamaJapan
  6. 6.Institute of Multidisciplinary Research for Advanced MaterialsTohoku UniversitySendaiJapan

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