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Structurally modified bacteriorhodopsin as an efficient bio-sensitizer for solar cell applications

  • T. C. Sabari Girisun
  • C. Jeganathan
  • N. Pavithra
  • S. Anandan
Original Article
  • 32 Downloads

Abstract

Structurally modified bacteriorhodopsin (BR) was prepared by simple surfactant treatment using Cetyl trimethylammonium bromide (cationic; CTAB), Sodium dodecyl sulphate (anionic; SDS) and Triton X-100 (nonionic; TX-100). In the UV–visible absorption spectrum, the characteristic absorption band of native BR at 560 nm is hyperchromically (CTAB, due to induced aggregation), bathochromically (SDS, BR solubilisation and partial unfolding) and hypsochromically (TX-100, BR monomerizes) shifted after chemical treatment and the structural modifications were further confirmed by Raman spectra. Theoretical calculations based on optical absorption support an enhancement of BR optical and electrical conductivity via structural modification. Bio-sensitized solar cells (BSSCs) with structurally altered BR as sensitizer were fabricated and their photovoltaic performance was measured. We obtained the maximum short-circuit photocurrent and photoelectric conversion efficiency with TX-100-treated BR (0.93 mA cm−2, 0.47%), with a quasi-Fermi level and a 124-ms lifetime of photogenerated electrons in TX-100-treated BR-sensitized BSSCs, two times higher than that observed in BSSCs with native BR. A single-diode equivalent circuit model reveals favorable BSSC parameters such as high reverse saturation current (I0 = 55 nA), low series resistance (Rs = 22.9 Ω) and high shunt resistance (Rsh = 3765.5 Ω) with TX-100-treated BR-based BSSCs. As TX-100 does not alter the BR carboxyl terminus during its monomerization, maximum anchoring to the BSSC occurs which results in enhanced photocurrent generation. Thus, monomerized BR-sensitized BSSCs with their excellent photovoltaic parameters suggest the possibility of replacing native BR with TX-100 BR and this opens up the possibility of reduced cost manufacture of bio-sensitized solar cells.

Keywords

Bacteriorhodopsin Structure modification BSSCs Enhanced photocurrent generation 

Notes

Acknowledgements

This work is financially supported by the Department of Science and Technology, Government of India under the DST-SERB Start up Grant Scheme (Grant no. SB/FTP/PS-038/2014) and RUSA (21/RUSA/2016). Mr. C.J. wishes to thank University Grants Commission, Government of India for providing UGC-SAP (RFMS) fellowship to carry out his research work (Grant no. F.4-1/2006(BSR)/7-197/2007).The author SA also wishes to thank Department of Technology (DST), India (EMR/2014/000009) for the financial support.

References

  1. Barnett SM, Dracheva S, Hendler RW, Levin IW (1996) Lipid-induced conformational changes of an integral membrane protein: an infrared spectroscopic study of the effects of Triton X-100 treatment on the purple membrane of Halobacterium halobium ET1001. Biochemistry 35:4558–4567CrossRefPubMedGoogle Scholar
  2. Bunaciu AA, Aboul-Enein HY, Hoang VD (2015) Raman spectroscopy for protein analysis. Appl Spectrosc Rev 50:377–386CrossRefGoogle Scholar
  3. Campion A, Terner J, El-Sayed MA (1977) Time-resolved resonance Raman spectroscopy of bacteriorhodopsin. Nature 265:659–661CrossRefPubMedGoogle Scholar
  4. Chang C-W, Chang C-H, Lu H-P, Wu T-K, Wei-Guang Diau E (2009) Fabrication and photovoltaic characterization of bio-sensitized solar cells using myoglobin-based sensitizers. J Nanosci Nanotechnol 9:1688–1695.  https://doi.org/10.1166/jnn.2009.SI05 CrossRefPubMedGoogle Scholar
  5. Cheng CW, Lee YP, Chu LK (2012) Study of the reactive excited-state dynamics of delipidated bacteriorhodopsin upon surfactant treatments. Chem Phys Lett 539:151–156.  https://doi.org/10.1016/j.cplett.2012.05.006 CrossRefGoogle Scholar
  6. Chu L-K, El-Sayed MA (2010a) Kinetics of the M-intermediate in the photocycle of bacteriorhodopsin upon chemical modification with surfactants. Photochem Photobiol 86:316–323.  https://doi.org/10.1111/j.1751-1097.2009.00666.x CrossRefPubMedGoogle Scholar
  7. Chu LK, El-Sayed MA (2010b) Bacteriorhodopsin O-state photocycle kinetics: a surfactant study. Photochem Photobiol 86:70–76CrossRefPubMedGoogle Scholar
  8. Chu L-K, Yen C-W, El-Sayed MA (2010) Bacteriorhodopsin-based photo-electrochemical cell. Biosens Bioelectron 26:620–626.  https://doi.org/10.1016/j.bios.2010.07.013 CrossRefPubMedGoogle Scholar
  9. Dracheva S, Bose S, Hendler RW (1996) Chemical and functional studies on the importance of purple membrane lipids in bacteriorhodopsin photocycle behavior. FEBS Lett 382:209–212.  https://doi.org/10.1016/0014-5793(96)00181-0 CrossRefPubMedGoogle Scholar
  10. Gushchin I, Reshetnyak A, Borshchevskiy V, Ishchenko A, Round E, Grudinin S (2011) Active state of sensory rhodopsin II: structural determinants for signal transfer and proton pumping. J Mol Biol 412:591–600.  https://doi.org/10.1016/j.jmb.2011.07.022 CrossRefPubMedGoogle Scholar
  11. Hampp N, Oesterhelt D (2008) Bacteriorhodopsin and its potential in technical applications. In: Protein science encyclopedia. Wiley-VCH Verlag GmbH & Co. KGaA.  https://doi.org/10.1002/9783527610754.bt02
  12. Hao S, Wu J, Huang Y, Lin J (2006) Natural dyes as photosensitizers for dye-sensitized solar cell. Sol Energy 80:209–214.  https://doi.org/10.1016/j.solener.2005.05.009 CrossRefGoogle Scholar
  13. Jeganathan C, Pavithra N, Sabari Girisun TC, Anandan S, Ashokkumar M (2016) Enhanced photocurrent generation in bacteriorhodopsin based bio-sensitized solar cells using gel electrolyte. J Photochem Photobiol B 162:208–212.  https://doi.org/10.1016/j.jphotobiol.2016.06.044 CrossRefGoogle Scholar
  14. Jeganathan C, Sabari Girisun TC, Rao SV, Thamaraiselvi K (2017) Variable ultrafast optical nonlinearity in bacteriorhodopsin achieved through simple chemical treatment. J Mater Sci 52:6866–6878.  https://doi.org/10.1007/s10853-017-0924-x CrossRefGoogle Scholar
  15. Jin Y, Friedman N, Sheves M, He T, Cahen D (2006) Bacteriorhodopsin (bR) as an electronic conduction medium: current transport through bR-containing monolayers. Proc Natl Acad Sci 103:8601–8606.  https://doi.org/10.1073/pnas.0511234103 CrossRefPubMedGoogle Scholar
  16. Krishnamani V, Hegde BG, Langen R, Lanyi JK (2012) Secondary and tertiary structure of bacteriorhodopsin in the SDS denatured state. Biochemistry 51:1051–1060CrossRefPubMedGoogle Scholar
  17. le Maire M, Champeil P, Møller JV (2000) Interaction of membrane proteins and lipids with solubilizing detergents. Biochimica et Biophysica Acta (BBA) Biomembranes 1508:86–111.  https://doi.org/10.1016/S0304-4157(00)00010-1 CrossRefGoogle Scholar
  18. Lee DC, Chapman D (1986) Infrared spectroscopic studies of biomembranes and model membranes. Biosci Rep 6:235–256CrossRefPubMedGoogle Scholar
  19. Li N, Pan N, Li D, Lin S (2013) Natural dye-sensitized solar cells based on highly ordered TiO2 nanotube arrays. Int J Photoenergy 2013:1–5.  https://doi.org/10.1155/2013/598753 CrossRefGoogle Scholar
  20. Liu SY, Kono M, Ebrey TG (1991) Effect of pH buffer molecules on the light-induced currents from oriented purple membrane. Biophys J 60:204–216.  https://doi.org/10.1016/S0006-3495(91)82044-6 CrossRefPubMedPubMedCentralGoogle Scholar
  21. London E, Khorana HG (1982) Denaturation and renaturation of bacteriorhodopsin in detergents and lipid-detergent mixtures. J Biol Chem 257:7003–7011PubMedGoogle Scholar
  22. Mohammadpour R, Janfaza S (2015) Efficient nanostructured biophotovoltaic cell based on bacteriorhodopsin as biophotosensitizer. ACS Sustain Chem Eng 3:809–813.  https://doi.org/10.1021/sc500617w CrossRefGoogle Scholar
  23. Ng KC, Chu L-K (2013) Effects of surfactants on the purple membrane and bacteriorhodopsin: solubilization or aggregation? J Phys Chem B 117:6241–6249.  https://doi.org/10.1021/jp401254j CrossRefPubMedGoogle Scholar
  24. Padrós E, Duñach M, Sabés M (1984a) Induction of the blue form of bacteriohodopsin by low concentrations of sodium dodecyl sulfate. Biochimica et Biophysica Acta (BBA)-Biomembranes 769:1–7CrossRefGoogle Scholar
  25. Padrós E, Duñach M, Sabés M (1984b) Induction of the blue form of bacteriohodopsin by low concentrations of sodium dodecyl sulfate. Biochimica et Biophysica Acta (BBA) Biomembranes 769:1–7.  https://doi.org/10.1016/0005-2736(84)90002-6 CrossRefGoogle Scholar
  26. Pavithra N, Asiri AM, Anandan S (2015) Fabrication of dye sensitized solar cell using gel polymer electrolytes consisting poly(ethylene oxide)-acetamide composite. J Power Sour 286:346–353.  https://doi.org/10.1016/j.jpowsour.2015.03.160 CrossRefGoogle Scholar
  27. Pramod Kumar S, Nagarale RK, Pandey SP, Rhee HW, Bhaskar B (2011) Present status of solid state photoelectrochemical solar cells and dye sensitized solar cells using PEO-based polymer electrolytes. Adv Nat Sci Nanosci Nanotechnol 2:023002CrossRefGoogle Scholar
  28. Sabari Girisun TC, Dhanuskodi S (2009) Linear and nonlinear optical properties of tris thiourea zinc sulphate single crystals. Cryst Res Technol 44:1297–1302.  https://doi.org/10.1002/crat.200900351 CrossRefGoogle Scholar
  29. Sánchez-Vergara ME, Alonso-Huitron JC, Rodriguez-Gómez A, Reider-Burstin JN (2012) Determination of the optical GAP in thin films of amorphous dilithium phthalocyanine using the Tauc and Cody models. Molecules 17:10000–10013CrossRefPubMedGoogle Scholar
  30. Seddon AM, Curnow P, Booth PJ (2004) Membrane proteins, lipids and detergents: not just a soap opera. Biochimica et Biophysica Acta (BBA) Biomembranes 1666:105–117.  https://doi.org/10.1016/j.bbamem.2004.04.011 CrossRefGoogle Scholar
  31. Shiu P-J, Ju Y-H, Chen H-M, Lee C-K (2013) Facile isolation of purple membrane from Halobacterium salinarum via aqueous-two-phase system. Protein Expr Purif 89:219–224.  https://doi.org/10.1016/j.pep.2013.03.011 CrossRefPubMedGoogle Scholar
  32. Subramaniam S, Marti T, Rosselet SJ, Rothschild KJ, Khorana HG (1991) The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles—selective reactivity of different photointermediates. Proc Natl Acad Sci USA 88:2583–2587.  https://doi.org/10.1073/pnas.88.6.2583 CrossRefPubMedGoogle Scholar
  33. Tan E, Birge RR (1996) Correlation between surfactant/micelle structure and the stability of bacteriorhodopsin in solution. Biophys J 70:2385–2395CrossRefPubMedPubMedCentralGoogle Scholar
  34. Thavasi V et al (2009) Study on the feasibility of bacteriorhodopsin as bio-photosensitizer in excitonic solar cell: a first report. J Nanosci Nanotechnol 9:1679–1687.  https://doi.org/10.1166/jnn.2009.SI07 CrossRefPubMedGoogle Scholar
  35. Thomas GJ Jr (1999) Raman spectroscopy of protein and nucleic acid assemblies. Annu Rev Biophys Biomol Struct 28:1–27CrossRefPubMedGoogle Scholar
  36. Wang Y, Wu J, Ming M, Zhao Y, Ding J (2014) Effects of Triton X-100 on proton transfer and in the photocycle of Archaerhodopsin 4 bioscience. Biotechnol Biochem 76:250–256.  https://doi.org/10.1271/bbb.110508 CrossRefGoogle Scholar
  37. Wang X, Huang H, Sun C, Huang F (2015) Structural analysis of bacteriorhodopsin solubilized by lipid-like phosphocholine biosurfactants with varying micelle concentrations. J Colloid Interface Sci 437:170–180.  https://doi.org/10.1016/j.jcis.2014.09.007 CrossRefPubMedGoogle Scholar
  38. Williams JC (1976) Doctor-blade process. In: Wang FFY (ed) Ceramic fabrication processes: treatise on materials science and technology, vol 9. Elsevier, pp 173–198.  https://doi.org/10.1016/B978-0-12-341809-8.50016-4
  39. Yamazaki E, Murayama M, Nishikawa N, Hashimoto N, Shoyama M, Kurita O (2007) Utilization of natural carotenoids as photosensitizers for dye-sensitized solar cells. Sol Energy 81:512–516.  https://doi.org/10.1016/j.solener.2006.08.003 CrossRefGoogle Scholar

Copyright information

© European Biophysical Societies' Association 2018

Authors and Affiliations

  • T. C. Sabari Girisun
    • 1
  • C. Jeganathan
    • 1
  • N. Pavithra
    • 2
  • S. Anandan
    • 2
  1. 1.Nanophotonics Laboratory, School of PhysicsBharathidasan UniversityTiruchirappalliIndia
  2. 2.Nanomaterials and Solar Energy Conversion Lab, Department of ChemistryNational Institute of TechnologyTiruchirappalliIndia

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