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European Biophysics Journal

, Volume 44, Issue 1–2, pp 27–36 | Cite as

Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica

  • Maria WallinEmail author
  • Jae-Hyeok Choi
  • Seong Oh Kim
  • Nam-Joon Cho
  • Martin Andersson
Original Paper

Abstract

Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greater stability. The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol % 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. A quartz crystal microbalance with dissipation monitoring was used to monitor the process of vesicle adsorption and tBLM self-assembly, and atomic force microscopy was performed to characterize the structural properties of the tBLM obtained. Whereas tether-containing lipid vesicles ruptured neither spontaneously nor as a result of osmotic shock, introduction of an amphipathic α-helical (AH) peptide induced vesicle rupture and subsequent tBLM formation. Taken together, our findings suggest that the AH peptide is an efficient means of rupturing vesicles of both simple and complex composition, and is, therefore, useful for formation of tBLMs on solid and mesoporous materials for applications in biotechnology.

Keywords

Mesoporous Tethered lipid bilayer membrane AH peptide Vesicle fusion 

Notes

Acknowledgments

Financial support was obtained from the Swedish Research Council, VR 2008-3660, the Materials Area of Advance Chalmers University of Technology, the National Research Foundation (NRF-NRFF2011-01), and the National Medical Research Council (NMRC/CBRG/0005/2012). The authors thank Sylvio Hass for assistance with SAXS measurements performed at Maxlab (Lund, Sweden) on beamline I911.

Supplementary material

249_2014_998_MOESM1_ESM.docx (45 kb)
Supplementary material 1 (DOCX 44 kb)

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Copyright information

© European Biophysical Societies' Association 2014

Authors and Affiliations

  • Maria Wallin
    • 1
    Email author
  • Jae-Hyeok Choi
    • 2
  • Seong Oh Kim
    • 2
  • Nam-Joon Cho
    • 2
    • 3
  • Martin Andersson
    • 1
  1. 1.Department of Chemical and Biological EngineeringChalmers University of TechnologyGöteborgSweden
  2. 2.School of Materials Science and EngineeringNanyang Technological UniversitySingaporeSingapore
  3. 3.School of Chemical and Biomolecular EngineeringNanyang Technological UniversitySingaporeSingapore

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