European Biophysics Journal

, Volume 43, Issue 10–11, pp 565–572 | Cite as

A permeability transition in liver mitochondria and liposomes induced by α,ω-dioic acids and Ca2+

  • Mikhail V. DubininEmail author
  • Victor N. Samartsev
  • Maxim E. Astashev
  • Alexey S. Kazakov
  • Konstantin N. Belosludtsev
Original Paper


The article examines the molecular mechanism of the Ca2+-dependent cyclosporin A (CsA)-insensitive permeability transition in rat liver mitochondria induced by α,ω-dioic acids. The addition of α,ω-hexadecanedioic acid (HDA) to Ca2+-loaded liver mitochondria was shown to induce a high-amplitude swelling of the organelles, a drop of membrane potential and the release of Ca2+ from the matrix, the effects being insensitive to CsA. The experiments with liposomes loaded with sulforhodamine B (SRB) revealed that, like palmitic acid (PA), HDA was able to cause permeabilization of liposomal membranes. However, the kinetics of HDA- and PA-induced release of SRB from liposomes was different, and HDA was less effective than PA in the induction of SRB release. Using the method of ultrasound interferometry, we also showed that the addition of Ca2+ to HDA-containing liposomes did not change the phase state of liposomal membranes—in contrast to what was observed when Ca2+ was added to PA-containing vesicles. It was suggested that HDA/Ca2+- and PA/Ca2+-induced permeability transition occurs by different mechanisms. Using the method of dynamic light scattering, we further revealed that the addition of Ca2+ to HDA-containing liposomes induced their aggregation/fusion. Apparently, these processes result in a partial release of SRB due to the formation of fusion pores. The possibility that this mechanism underlies the HDA/Ca2+-induced permeability transition of the mitochondrial membrane is discussed.


Liver mitochondria α,ω-Dioic acids Ca2+ Liposome Lipid pore Membrane fusion 



Cyclosporin A


α,ω-Hexadecanedioic acid


Palmitic acid


α,ω-Tetradecanedioic acid


Sulforhodamine B


Cation tetraphenylphosphonium


Large unilamellar vesicle


Triton X-100





We are grateful to Dr. Alexey Agafonov for fruitful discussions. This study was supported by the Ministry of Education and Science of the Russian Federation (Project No. 1365) by the Government of RF (Project No. 14.Z50.31.0028) and by grants from the Russian Foundation for Basic Research (14-04-00688-a, 12-04-00430-a; 14-34-50380).


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Copyright information

© European Biophysical Societies' Association 2014

Authors and Affiliations

  • Mikhail V. Dubinin
    • 1
    • 2
    Email author
  • Victor N. Samartsev
    • 1
  • Maxim E. Astashev
    • 2
    • 3
  • Alexey S. Kazakov
    • 4
  • Konstantin N. Belosludtsev
    • 2
  1. 1.Mari State UniversityYoshkar-OlaRussia
  2. 2.Institute of Theoretical and Experimental Biophysics RASPushchinoRussia
  3. 3.Institute of Cell Biophysics RASPushchinoRussia
  4. 4.Institute for Biological Instrumentation RASPushchinoRussia

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