European Biophysics Journal

, Volume 42, Issue 11–12, pp 811–818 | Cite as

Ribosylation of bovine serum albumin induces ROS accumulation and cell death in cancer line (MCF-7)

  • Mohd Shahnawaz Khan
  • Sourabh Dwivedi
  • Medha Priyadarshini
  • Shams Tabrez
  • Maqsood Ahmed Siddiqui
  • Haseeb Jagirdar
  • Abdulrahman M. Al-Senaidy
  • Abdulaziz A. Al-Khedhairy
  • Javed Musarrat
Original Paper

Abstract

Formation of advanced glycation end products (AGE) is crucially involved in the several pathophysiologies associated with ageing and diabetes, for example arthritis, atherosclerosis, chronic renal insufficiency, Alzheimer’s disease, nephropathy, neuropathy, and cataracts. Because of devastating effects of AGE and the significance of bovine serum albumin (BSA) as a transport protein, this study was designed to investigate glycation-induced structural modifications in BSA and their functional consequences in breast cancer cell line (MCF-7). We incubated d-ribose with BSA and monitored formation of d-ribose-glycated BSA by observing changes in the intensity of fluorescence at 410 nm. NBT (nitro blue tetrazolium) assay was performed to confirm formation of keto-amine during glycation. Absorbance at 540 nm (fructosamine) increased markedly with time. Furthermore, intrinsic protein and 8-anilino-1-naphthalenesulfonate (ANS) fluorescence revealed marked conformational changes in BSA upon ribosylation. In addition, a fluorescence assay with thioflavin T (ThT) revealed a remarkable increase in fluorescence at 485 nm in the presence of glycated BSA. This suggests that glycation with d-ribose induced aggregation of BSA into amyloid-like deposits. Circular dichroism (CD) study of native and ribosylated BSA revealed molten globule formation in the glycation pathway of BSA. Functional consequences of ribosylated BSA on cancer cell line, MCF-7 was studied by MTT assay and ROS estimation. The results revealed cytotoxicity of ribosylated BSA on MCF-7 cells.

Keywords

Glycation Bovine serum albumin Cytotoxicity Fluorescence Circular dichroism 

Notes

Acknowledgment

The authors extend their appreciation to the Deanship of Scientific Research at KSU for funding this work through research group project number RGP-VPP-215.

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Copyright information

© European Biophysical Societies' Association 2013

Authors and Affiliations

  • Mohd Shahnawaz Khan
    • 1
  • Sourabh Dwivedi
    • 2
  • Medha Priyadarshini
    • 3
  • Shams Tabrez
    • 5
  • Maqsood Ahmed Siddiqui
    • 2
  • Haseeb Jagirdar
    • 1
  • Abdulrahman M. Al-Senaidy
    • 1
  • Abdulaziz A. Al-Khedhairy
    • 2
  • Javed Musarrat
    • 4
  1. 1.Department of Biochemistry, College of ScienceKing Saud UniversityRiyadhKingdom of Saudi Arabia
  2. 2.DNA Research Chair, Department of ZoologyKing Saud UniversityRiyadhKingdom of Saudi Arabia
  3. 3.Department of Medicine, Division of Endocrinology, Metabolism and Molecular MedicineNorthwestern University Feinberg School of MedicineChicagoUSA
  4. 4.Department of Agricultural MicrobiologyAligarh Muslim UniversityAligarhIndia
  5. 5.King Fahd Medical Research CenterKing Abdulaziz University JeddahSaudi Arabia

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