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European Biophysics Journal

, Volume 42, Issue 8, pp 613–620 | Cite as

Mechanical double layer model for Saccharomyces Cerevisiae cell wall

  • Ruben Mercadé-PrietoEmail author
  • Colin R. Thomas
  • Zhibing Zhang
Original Paper

Abstract

The elastic modulus of the Baker’s yeast (Saccharomyces cerevisiae) cell wall reported in studies using atomic force microscopy (AFM) is two orders of magnitude lower than that obtained using whole cell compression by micromanipulation. Using finite element modelling, it is shown that Hertz-Sneddon analysis cannot be applied to AFM indentation data for single layer core–shell structures. In addition, the Reissner solution for shallow homogeneous spheres is not appropriate for thick walls such as those of yeast cells. In order to explain yeast compression measurements at different length scales, a double layer wall model is presented considering a soft external layer composed of mannoproteins, and a stiff inner layer of β-glucan fibres and chitin. Under this model, previous AFM studies using sharp indenters provide reasonable estimates of the external layer elastic modulus, while micromanipulation provides the total stiffness of the cell wall. Data from both measurements are combined to estimate the mechanical properties of the inner stiff layer.

Keywords

Elasticity Compression Indentation Yeast Finite element modelling 

Notes

Acknowledgments

The authors gratefully acknowledge financial support from the Engineering and Physical Sciences Research Council (EPRSC), UK, through grant number EP/F068395/1.

Supplementary material

249_2013_909_MOESM1_ESM.doc (1.6 mb)
Supplementary material 1 (DOC 1628 kb)

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Copyright information

© European Biophysical Societies' Association 2013

Authors and Affiliations

  • Ruben Mercadé-Prieto
    • 1
    • 2
    Email author
  • Colin R. Thomas
    • 1
  • Zhibing Zhang
    • 1
  1. 1.School of Chemical EngineeringUniversity of BirminghamBirminghamUK
  2. 2.Chemical Engineering Innovation LabSoochow UniversitySuzhouChina

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