Characterization of new DOPC/DHPC platform for dermal applications
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Systems formed by mixtures of the phospholipids dioleoylphosphatidylcholine (DOPC) and dihexanoylphosphatidylcholine (DHPC) were characterized by use of differential scanning calorimetry, small angle X-ray scattering and two electron-microscopy techniques, freeze fracture electron microscopy and cryogenic transmission electron microscopy. These techniques allowed for the determination of the size, morphology, structural topology, self-assembly and thermotropic behavior of the nanostructures present in the mixtures. The interaction between the two phospholipids provides curvatures, irregularities and the increase of thickness and flexibility in the membrane. These effects led to the formation of different aggregates with a differential distribution of both phospholipids. The effect of these systems on the skin in vivo was evaluated by measurement of the biophysical skin parameters. Our results show that the DOPC/DHPC application induces a decrease in the permeability and in the hydration of the tissue. These effects in vivo are related to different microstructural changes promoted by these systems in the skin in vitro, published in a recent work. The fundamental biophysical analyses of DOPC/DHPC systems contribute to our understanding of the mechanisms that govern their interaction with the skin.
KeywordsDioleoyl-glycero-phosphatidylcholine Dihexanoyl-glycero-phosphatidylcholine Lipid aggregates Skin parameters
Freeze fracture electron microscopy
Small-angle X-ray scattering
Differential scanning calorimetry
Cryo-transmission electron microscopy
Trans epidermal water loss
The authors wish to thank Ramon Pons, Jaume Caelles, Josep Carrilla and Rocío Vicente for expert technical assistance. M. Cócera is funded by the JAE-DOC program from CSIC (co-funded by FSE). This work was supported by funds from CICYT (CTQ 2010-16964) and from Generalitat de Catalunya (2009 SGR 1212).
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