Depth-sensing analysis of cytoskeleton organization based on AFM data
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Atomic force microscopy is a common technique used to determine the elastic properties of living cells. It furnishes the relative Young’s modulus, which is typically determined for indentation depths within the range 300–500 nm. Here, we present the results of depth-sensing analysis of the mechanical properties of living fibroblasts measured under physiological conditions. Distributions of the Young’s moduli were obtained for all studied cells and for every cell. The results show that for small indentation depths, histograms of the relative values of the Young’s modulus described the regions rich in the network of actin filaments. For large indentation depths, the overall stiffness of a whole cell was obtained, which was accompanied by a decrease of the modulus value. In conclusion, the results enable us to describe the non-homogeneity of the cell cytoskeleton, particularly, its contribution linked to actin filaments located beneath the cell membrane. Preliminary results showing a potential application to improve the detection of cancerous cells, have been presented for melanoma cell lines.
KeywordsCell biomechanics Atomic force microscopy Depth-sensing analysis Cell cytoskeleton
Atomic force microscopy
Minimum essential medium
Full width of the distribution taken at half height
This work was partially supported by the project SMW (Single Molecule Workstation), grant agreement number 213717 (NMP4-SE-2008-213717), and by the projects of the Polish Ministry of Science and Higher Education numbers: N-N202-285738 (O.K.) and N-N402-47133 (M.F.).
- Goetz JG, Minguet S, Navarro-Lerida I, Lazcano JJ, Samaniego R, Calvo E, Tello M, Osteo-Ibanez T, Pellinen T, Echarri A, Cerezo A, Klein-Szanto AJP, Garcia R, Kelly PJ, Sanchez-Mateo P, Cukierman E, Del Pozo MA (2011) Biomechanical remodelling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis. Cell 146:148–163PubMedCrossRefGoogle Scholar
- Guck J, Schinkinger S, Lincoln B, Wottawah F, Ebert S, Romeyke M, Lenz D, Erickson HM, Ananthakrishnan R, Mitchell D, Käs J, Ulvick S, Bilby S (2005) Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophys J 88:3689–3698PubMedCrossRefGoogle Scholar
- Zhou J, Giannakakou P (2005) Targeting microtubules for cancer chemotherapy. Curr Med Chem 5:65–71Google Scholar