Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
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Abstract
Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active volume, and intracellular osmolarity have been investigated for decades, but a consistent rigorous parameterization of these concepts is lacking. Here, we subjected several data sets of minimum volume measurements in yeast obtained after hyper-osmotic shock to a thermodynamic modeling framework. We estimated parameters for several relevant biophysical cell properties and tested alternative hypotheses about these concepts using a model discrimination approach. In accordance with previous reports, we estimated an average initial turgor of 0.6 ± 0.2 MPa and found that turgor becomes negligible at a relative volume of 93.3 ± 6.3% corresponding to an osmotic shock of 0.4 ± 0.2 Osm/l. At high stress levels (4 Osm/l), plasmolysis may occur. We found that the volumetric elastic modulus, a measure of cell wall elasticity, is 14.3 ± 10.4 MPa. Our model discrimination analysis suggests that other thermodynamic quantities affecting the intracellular water potential, for example the matrix potential, can be neglected under physiological conditions. The parameterized turgor models showed that activation of the osmosensing high osmolarity glycerol (HOG) signaling pathway correlates with turgor loss in a 1:1 relationship. This finding suggests that mechanical properties of the membrane trigger HOG pathway activation, which can be represented and quantitatively modeled by turgor.
Keywords
Turgor Cell wall elasticity Volumetric elastic modulus High osmolarity glycerol (HOG) signaling Plasmolysis Model discrimination YeastIntroduction
All living cells maintain an osmotic pressure gradient between their interior and the extracellular environment. This gradient is counterbalanced by a hydrostatic pressure, called turgor, which is especially prominent in plants and unicellular organisms, most notably fungi. The concept of turgor has been debated for many years (Burström 1971). It is recognized that turgor plays a vital role in growth (Cosgrove 1981; Zimmermann 1978; Marechal and Gervais 1994), cell structure (Cosgrove 1993; Morris et al. 1986; Zhongcan and Helfrich 1987; Munns et al. 1983), and membrane transport processes (Zimmermann 1978; Lande et al. 1995; Kamiya et al. 1963; Soveral et al. 2008) and that it may be sensed by the cells as an indicator of external osmotic changes (Tamas et al. 2000; Coster et al. 1976; Schmalstig and Cosgrove 1988; Reiser et al. 2003). Therefore, quantifying cell wall and membrane properties, and thus the resulting turgor pressure, has been the aim of numerous studies (Cosgrove 1981; Morris et al. 1986; Munns et al. 1983; Kamiya et al. 1963; Coster et al. 1976; Smith et al. 1998, 2000a, b, c; Marechal et al. 1995; Gervais et al. 1996a; Cosgrove 2000). Parameterized models of turgor pressure, based on cell wall and membrane properties are especially important for quantitative mathematical models describing the interdependence between water transport and signaling processes (Schaber and Klipp 2008; Klipp et al. 2005; Gennemark et al. 2006; Kargol and Kargol 2003a, 2003).
The concepts of turgor, cell wall elasticity, osmotically active volume and intracellular osmolarity have never been quantified and parameterized together in a consistent framework, nor challenged by alternative concepts (Smith et al. 2000c; Martinez de Maranon et al. 1996; Gervais et al. 1996b). Moreover, the few reports in which turgor and/or cell wall elasticity were quantitatively determined used non-physiological experimental conditions. For example, one study used pre-stressed cells (Marechal et al. 1995) and in two others, the cells were suspended in distilled water before volume measurements (Smith et al. 2000b, c).
The objective of this study was to derive parameterized models of turgor and other biophysical properties of the cell, for example the volumetric elastic modulus, in Saccharomyces cerevisiae. We developed a modeling framework that includes the concepts of turgor pressure, intra and extracellular osmotic pressure, and membrane and volume properties and, for the first time, also other thermodynamic quantities, for example the matrix potential, in a consistent manner. By reformulating sub-modules while keeping the overall framework consistent, we could test several hypotheses describing the relationship between cell volume and changes in the intracellular water potential. Verification of these hypotheses was based on four independent data sets. To ensure a wide applicability of the selected model, the conditions for generating each data set were slightly different. We also correlated cell volume measurements with Hog1 phosphorylation levels and Hog1-GFP nuclear localization to investigate the physiological consequences of turgor loss under hyper-osmotic stress in a quantitative fashion. Hog1 is the yeast p38 stress-activated protein kinase whose phosphorylation level and subcellular localization are controlled by an osmo-sensing signaling pathway (Hohmann 2002). We found a 1:1 relationship between turgor loss and HOG pathway activation. A loss of turgor relates to HOG pathway activation such that when turgor becomes zero because of application of a hyperosmotic solution the HOG pathway is maximally activated. This finding suggests that the extent of HOG pathway activation is directly linked to some mechanical property of the membrane, which can be represented and quantitatively modeled by the loss of turgor pressure.
Materials and methods
Summary of quantities used in the main text
Quantity | Unit | Description |
---|---|---|
V _{ap} | % initial volume | Apparent cell volume, enclosed by the cell wall |
V _{ap} ^{0} | % initial volume | Initial V _{ap} |
V _{ap} ^{min} | % initial volume | Minimal V _{ap} attained after hyperosmotic shock |
V _{m} | % initial volume | Cell volume, enclosed by the plasma membrane |
V _{os} | % initial volume | Osmotically active volume |
V _{pl} | % initial volume | Periplasmic volume. We tested three alternative models of V _{pl} as a function of c _{stress} ^{e} (see “Supplementary material”) |
V _{b} | % initial volume | Solid cytosolic volume |
V _{m} ^{P=0} | % initial volume | Volume, when turgor becomes zero |
V _{m} ^{τ} | % initial volume | Volume below which negative hydrostatic or matrix potential effects are important |
V _{ap} ^{pl} | % initial volume | Apparent volume where plasmolysis occurs |
c _{0} ^{ i } | Osm/l | Initial internal osmolarity. |
c _{0} ^{e} | Osm/l | Initial external osmolarity |
c _{stress} ^{e} | Osm/l | Applied osmotic stress |
c _{P=0} ^{e} | Osm/l | c _{stress} ^{e} corresponding to V _{m} ^{P=0} |
c _{τ} ^{e} | Osm/l | c _{stress} ^{e} corresponding to V _{m} ^{τ} |
c _{pl} ^{e} | Osm/l | c _{stress} ^{e} corresponding to V _{ap} ^{pl} |
P _{0} | MPa | Initial turgor at the initial turgid cell volume of 100% |
\( P_{{V_{\text{ap}}^{\min } }} \) | MPa | Turgor at V _{ap} ^{min} . In general, there are two alternatives, i.e., the one-sided model (Eq. S7 in “Supplementary material”) and the two-sided model (Eq. S8 in “Supplementary material”) (Fig. 2) |
ɛ | MPa | Volumetric elastic modulus, describing the elasticity of the cell wall, relating relative volume change to hydrostatic pressure, i.e., turgor |
ɛ _{τ} | MPa | Proportionality factor, relating relative volume change to the matrix potential or other effects negatively influencing the intracellular water potential |
R | J K^{−1} mol^{−1} | Gas constant |
T | K | Temperature |
α _{PC} | Dimensionless | Conversion factor relating pressure to osmolarity, e.g., for relating MPa and Osm/l it is 10^{−3} |
Modeling framework
Turgor pressure and matrix potential
We tested Eq. 3 against the data, with and without the term for matrix potential, which we refer to as the one-sided and the two-sided models, respectively. We also tested three hypotheses regarding the size of the periplasmic volume V _{pl}, (a) V _{pl} = 0, (b) V _{pl} > 0 with a constant apparent volume V _{ap} ^{pl} (Fig. 1; Eq. S10 in “Supplementary material”), and (c) V _{pl} > 0 with a variable apparent volume V _{ap} ^{pl} (Eq. S11 in “Supplementary material”).
Inserting Eq. 3 into Eq. 2 yields an implicit expression for V _{ap} ^{min} that depends on various parameters of the specific model for turgor, matrix effect, and periplasmic volume. When a solution exists, this solution is unique (see “Supplementary material”).
Model discrimination
The fitting was performed by differential evolution, a global optimization method (Storn and Price 1997), and the models were ranked according to the AICc (Eq. 4). Additionally, for the best model, we performed a Monte–Carlo analysis by re-sampling the data a hundred times assuming a normal distribution with the respective standard deviations for each data point. In this way, means and confidence intervals for the estimated parameters could be obtained.
Data sets
Data sets used for model discrimination
No. | Method | Strain | Medium | Agent | °C | Growth | c _{0} ^{e} (Osm/l) | # n | Refs. |
---|---|---|---|---|---|---|---|---|---|
1 | Particle sizer | W303 | YPD | NaCl | 30 | Early log | 0.26 | 16 | This study |
2 | Single cells bright field | BY4741 | YNB | NaCl | 20 | Log | 0.27 | 11 | This study |
3 | Single cells fluorescence | W303 | SD | NaCl | 30 | Log | 0.25 | 13 | This study |
4 | Single cells bright field | CBS1171 | Wickerham | Glycerol | 25 | Stationary | 0.86 | 15 | Marechal et al. (1995) |
Results
Parameter fitting and model selection predicts turgor pressure and other biophysical cell properties
Results of parameter fits of Eq. 2 for the best selected model according to the AICc
Data set 1 | Data set 2 | Data set 3 | Data set 4 | ||
---|---|---|---|---|---|
# | 2 | 2 | 2 | 4 | Selected model |
\( P_{{V_{\text{m}}^{\min } }} \) | One-sided | One-sided | One-sided | One-sided | |
V _{pl} | 0 | 0 | 0 | Constant V _{ap} ^{pl} | |
k | 3 | 3 | 3 | 4 | |
V _{b} | 49.0 ± 3.4 | 40.2 ± 5.5 | 33.0 ± 4.8 | 40.1 ± 11.1 | Estimated parameters |
P _{0} | 0.5 ± 0.1 | 0.9 ± 0.2 | 0.5 ± 0.1 | 3.4 ± 1.0 | |
ɛ | 25.8 ± 7.0 | 5.7 ± 2.0 | 11.4 ± 2.1 | 28.6 ± 11.3 | |
V _{ap} ^{pl} | 65.0 ± 5.9 | ||||
c _{0} ^{ i } | 0.4 ± 0.1 | 0.6 ± 0.1 | 0.5 ± 0.0 | 2.2 ± 0.4 | Derived parameters |
V _{m} ^{P=0} | 98.2 ± 3.1 | 86.1 ± 6.3 | 95.5 ± 6.8 | 88.9 ± 10.8 | |
c _{P=0} ^{e} | 0.2 ± 0.1 | 0.5 ± 0.2 | 0.2 ± 0.1 | 1.9 ± 1.9 | |
c _{pl} ^{e} | 4.4 ± 2.2 |
One-sided turgor model is most appropriate for physiological stress conditions
The three data sets produced for this study were all fitted best by the one-sided turgor model based on Eqs. 1 and 3, where only the positive turgor pressure plays a role (Fig. 2). Even though this is the generally accepted model (Cosgrove 1981; Marechal and Gervais 1994; Klipp et al. 2005; Gennemark et al. 2006; Meikle et al. 1988), we tested it with six other models, because to our knowledge it has never been challenged by other models, nor rigorously fitted to data. Thus, we show for the first time that effects resisting cell shrinkage, for example the matrix potential or mechanical forces, can be neglected under physiological conditions. Consequently, when the turgor becomes zero after an external osmotic shock of c _{P=0} ^{e} (Table 1; Fig. 3), the measured apparent volume V _{ap} is well predicted by the membrane enclosed volume V _{m} according to the van’t Hoff’s equation (Eq. S6 in “Supplementary material”). This means that the cell wall tightly follows the shrinkage of the membrane enclosed volume. Data set 4, which was collected by applying extreme stress levels, also favored the one-sided turgor model. However, at 4 Osm/l plasmolysis was predicted, with a constant minimal apparent volume V _{ap} ^{pl} of 65%.
Estimated biophysical cell properties are similar between experiments
Parameters estimated on the basis of the datasets give a consistent picture of important biophysical cell properties. For data sets 2 and 3, where single cell information was available, we distinguished between small and large cells and repeated the analysis to test whether cell size had an effect on the results. We found no significant differences between small and large cells with regard to the estimated parameters, which is in agreement with earlier reports (Martinez de Maranon et al. 1996). The solid volume V _{b} was estimated to be between 33 and 49% of the initial cell volume, which also corresponds to earlier reports (Marechal et al. 1995; Meikle et al. 1988). The initial turgor estimated on the basis of the three data sets with similar experimental conditions (data sets 1–3) was 0.6 ± 0.2 MPa on average, which confirmed results from earlier studies (Smith et al. 2000c; Gervais et al. 1996a; Meikle et al. 1988). The estimated membrane rigidity ɛ was the parameter that varied the most between experiments and it turned out to be a sensitive, poorly determined parameter, which is reflected by the relatively high standard deviations. However, at least for the three directly comparable data sets 1–3, the results were similar, giving an average volumetric elastic modulus of 14.3 ± 10.4 MPa ranging from 5.7 to 25.8 MPa.
On the basis of our general modeling framework and the estimated parameters, we could derive several other interesting cell properties. The initial osmolarity within the cell c _{0} ^{ i } was estimated to be up to more than twice the osmolarity of the outside medium. Thus, apparently, yeast can maintain substantial osmotic gradients between the inside and outside of the cell, which are equilibrated by turgor. The volume at which turgor becomes zero was estimated to be of 93.3 ± 6.3% on average for data sets 1–3. The corresponding external shock for the salt experiments was 0.4 ± 0.2 Osm/l, corresponding to a salt concentration of roughly 0.2 M NaCl. As this is within the range of concentrations in which saturation of HOG pathway activation is observed (Maeda et al. 1995), we hypothesized that the initial HOG pathway response may be correlated with turgor.
Loss of turgor correlates with HOG pathway activation
We fitted a linear relationship between HOG pathway activation and relative turgor for the three different parameterized models, corresponding to data sets 1–3, and the two different HOG pathway activation data sets (black lines in Fig. 5) by a weighted orthogonal regression. Our null hypothesis claimed a direct 1:1 linear relationship, i.e., H_{0}: a + bx, with (a, b) = (100, −1) (gray lines in Fig. 5). To test H_{0} we computed confidence regions for the estimated parameter pairs (a, b) by a Monte–Carlo analysis. As can be seen from the insets in Fig. 5, in all cases the hypothesized relationship (gray points) was within the 95% confidence region of the actual estimated parameter pair. Thus, the null hypothesis could not be rejected. Moreover, the Kendall-rank correlation coefficient (Kendall and Gibbons 1990) was highly significant in all cases (P < 0.01).
Discussion
Parameterized models of biophysical and mechanical cell properties become increasingly important for quantitative and predictive descriptions of cellular behavior. Our modeling framework, which included the concepts of turgor, membrane elasticity, intracellular osmolarity, osmotically active volume, non-turgid volume, and solid cytosolic volume in a coherent and thermodynamically accurate way, enabled us to parameterize all these concepts consistently, by fitting the relevant parameters to experimental data. In addition, it enabled us to discriminate between alternative hypotheses for turgor and volume changes by including alternative sub-models within the general framework. Even though the data sets were based on very different techniques, the overall conclusions are strikingly similar indicating that the proposed mathematical description is both accurate and universal.
We confirmed previous reports, which suggested an initial turgor pressure of around 0.6 MPa (Smith et al. 2000b; Meikle et al. 1988). Turgor pressures reported for yeast differ substantially. Part of this variation might be because they have never been rigorously fitted to data. In our study, we obtained similar results for data sets 1–3. However, the initial turgor estimated from data set 4 was sixfold higher. We assume that this discrepancy is caused by different growth phases of yeast cells employed in the experiments. This explanation is supported by earlier reports, in which differences in turgor between cells in different physiological states were observed. Specifically, a higher turgor pressure was reported for cells in stationary phase (Smith et al. 2000c; Martinez de Maranon et al. 1996). After examining different possible explanations (Eq. 3), we confirmed the hypothesis that turgor pressure is significant only above a specific volume threshold, below which it can be neglected, as previously proposed (Cosgrove 1981; Marechal and Gervais 1994; Klipp et al. 2005; Gennemark et al. 2006; Meikle et al. 1988). Other forces potentially affecting the water potential, and therefore water flux within the cell, can be neglected under the studied conditions of osmotic shock up to 1.5 M NaCl.
We found values for the volumetric elastic modulus ε between 6 and 29 MPa. We could not find any published values of ε for yeast. In a recent study, where yeast cells were subjected to mechanical compression, values of Young’s modulus E for the cell wall of around 110 MPa were reported (Smith et al. 2000a, c). Unfortunately, we could not relate these values to our volumetric elastic modulus, because those values for Young’s modulus E were derived assuming a Poisson’s ratio ν of 0.5 and, hence, the formula E = 3ε (1 − 2ν) was not applicable. We noted that cells in stationary phase (data set 4) are estimated to be more rigid, i.e., have a higher volumetric elastic modulus, but to a much lesser extent than in Smith et al. (2000c). The cells used in data set 1 were grown to saturation, i.e., beyond proliferation, before they were re-suspended in fresh medium for 1 h before measurements. It is possible that they were still recovering from saturation phase and therefore had higher cell wall rigidity than cells from data sets 2 and 3, which were measured at logarithmic growth phase. This reflects that ε is a function of the physiological state of the cell. Thus, when turgor is being modeled over longer time intervals than in this study, the assumption of ε being constant might be compromised.
Our modeling results support the notion of a rather elastic cell wall, because the cell wall tightly follows the cell membrane on shrinkage, and plasmolysis only occurs under extremely high stresses. There is experimental evidence from mammalian cells that our model of an elastic membrane/cell wall surrounding a viscous medium is an over-simplification (Wang et al. 2001). However, our model proved sufficient to explain our data. More complicated models were not supported by the data in respect of the AICc. It is known that upon osmotic shock the actin cytoskeleton in yeast de-polymerizes (Brewster and Gustin 1994). This is in line with our results in which effects of the skeleton, which we included into the model of the matrix potential, were refuted by the model discrimination. A recent compression study of mammalian cells also ruled out the cytoskeleton as being responsible for the increase in cell stiffness as the volume decreases (Zhou et al. 2009).
Other authors identified the concentration at which plasmolysis occurs as approximately 1 Osm/l (Arnold and Lacy 1977), but under different experimental conditions. The predicted plasmolysis point of 65% in data set 4 was higher than the lowest volumes measured in the other data sets (44–54%, Fig. 3). Therefore, cells might have been at the threshold of plasmolysis in those experiments. Because no data were collected at higher stress levels this effect was not distinguishable by model selection.
For the first time, we provide evidence that there is direct link between loss of turgor upon osmotic shock and HOG pathway activation, not only qualitatively but also quantitatively. Moreover, the hypothesis of a direct 1:1 relationship between turgor loss and HOG pathway activation could not be rejected. The physical basis of such a direct relationship remains elusive, but could for example be a change in Sln1 and Sho1 membrane protein conformation as a function of membrane stretch or other mechanical forces that relate to turgor.
Notes
Acknowledgments
We thank Patrick Gervais for providing a digital version of data set 4, Rosie Perkins, Javier Macia, and Karlheinz Schaber for useful suggestions on the manuscript, and Martina Fröhlich for measurement support. This work was supported via several projects funded by the European Commission: QUASI (Contract No. 503230 to SH, EK, FP and MP), CELLCOMPUT (Contract No. 043310 to SH, EK and FP), UNICELLSYS (Contract No. 201142 to SH, EK, FP, MP and MG), SYSTEMSBIOLOGY (Contract No. 514169 to SH and EK), and AMPKIN (Contract No. 518181 to SH and MG). In addition work was funded by grants from the Swedish Foundation for Strategic Research SSF (Bio-X to MG), the Swedish Research Council (project grants to SH and MG), the Carl Trygger Foundation (to MG), the Science Faculty, University of Gothenburg (to SH and MG), and the Swiss systemsX.ch (to MP).
Open Access
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Supplementary material
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