Structured illumination microscopy of a living cell
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Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.
KeywordsStructured illumination microscopy Live-cell imaging Resolution improvement
This work was funded by the Medical Research Council, Carl Zeiss MicroImaging GmbH and the International Agency for Atomic Energy.
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