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European Biophysics Journal

, Volume 39, Issue 1, pp 191–199 | Cite as

Novel effects of methyl viologen on photosystem II function in spinach leaves

  • Da-Yong Fan
  • Husen Jia
  • James Barber
  • Wah Soon ChowEmail author
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Abstract

Methyl viologen (MV) is a well-known electron mediator that works on the acceptor side of photosystem I. We investigated the little-known, MV-induced inhibition of linear electron flow through photosystem II (PS II) in spinach-leaf discs. Even a low [MV] decreased the (1) average, light-adapted photochemical efficiency of PS II traps, (2) oxidation state of the primary quinone acceptor QA in PS II during illumination, (3) photochemical efficiency of light-adapted open PS II traps, (4) fraction of absorbed light energy dissipated constitutively in a light-independent manner or as chlorophyll (Chl) a fluorescence emission, (5) Chl a fluorescence yield corresponding to dark-adapted open reaction-center traps (F o) and closed reaction-center traps (F m), and (6) half-time for re-oxidation of Q A in PS II after a single-turnover flash. These effects suggest that the presence of MV accelerates various “downhill” electron-transfer steps in PS II. Therefore, when using the MV to quantify cyclic electron flow, the inhibitory effect of MV on PS II should be taken into account.

Keywords

Cyclic electron flow Exciton-radical pair equilibrium Linear electron flow Methyl viologen Photosystem II 

Abbreviations

ATP

Adenosine triphosphate

CEF

Cyclic electron flow

Chl

Chlorophyll

Cyt

Cytochrome

D1, D2 protein

psbA, B gene product, respectively

Fo, Fm

Chl fluorescence corresponding to open and closed PS II traps in the dark-adapted state, respectively

Fo′, Fm

Chl fluorescence corresponding to open and closed PS II traps in the light-adapted state, respectively

Fv

Variable Chl a fluorescence in the light-adapted state (=F m′  F o)

LEF

Linear electron flow

MV

Methyl viologen

NADP+

Oxidized nicotinamide adenine dinucleotide phosphate

Φf,D

The fraction of absorbed light either dissipated constitutively as heat in a light-independent manner or emitted as Chl a fluorescence

ΦNPQ

The fraction of absorbed light partitioned as heat dissipation in a light-dependent manner

ΦPS II

The average quantum yield of PS II photochemistry in the light

P680, P700

Special Chl pair in the PS II, I reaction centers, respectively

PC

Plastocyanin

PQ

Plastoquinone

Ph

Pheophytin

PS I, II

Photosystem I, II, respectively

QA, QB

Primary, secondary quinone acceptor in PS II, respectively

qP

Oxidation state of QA

Notes

Acknowledgments

We gratefully acknowledge the support of this work by an Australian Research Council (Grant DP0664719) to W.S.C. and J.B., and by The National Natural Science Foundation of China (No. 30770346) and an Endeavour Fellowship (both to D.-Y.F.).

References

  1. Bendall DS, Manasse R (1995) Cyclic photophosphorylation and electron transport. Biochim Biophys Acta 1229:23–38. doi: 10.1016/0005-2728(94)00195-B CrossRefGoogle Scholar
  2. Björkman O, Demmig B (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origin. Planta 170:489–504. doi: 10.1007/BF00402983 CrossRefGoogle Scholar
  3. Chow WS, Hope AB (2004) Electron fluxes through photosystem I in cucumber leaf discs probed by far-red light. Photosynth Res 81:77–89. doi: 10.1023/B:PRES.0000028396.83954.36 CrossRefPubMedGoogle Scholar
  4. Evans AG, Evans JC, Baker MW (1977) Electron spin resonance study of the dimerization equilibrium of the radical cation of 1, 1′-diethyl-4, 4′-bipyridylium diiodide in methanol. J Am Chem Soc 99:5882–5884. doi: 10.1021/ja00460a006 CrossRefGoogle Scholar
  5. Fan D-Y, Nie Q, Hope AB, Hillier W, Pogson BJ, Chow WS (2007) Quantification of cyclic electron flow around photosystem I in spinach leaves during photosynthetic induction. Photosynth Res 94:347–357. doi: 10.1007/s11120-006-9127-z CrossRefPubMedGoogle Scholar
  6. Genty B, Briantais J-M, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92Google Scholar
  7. Hendrickson L, Furbank RT, Chow WS (2004) A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. Photosynth Res 82:73–81. doi: 10.1023/B:PRES.0000040446.87305.f4 CrossRefPubMedGoogle Scholar
  8. Jia H, Oguchi R, Hope AB, Barber J, Chow WS (2008) Differential effects of severe water stress on linear and cyclic electron fluxes through photosystem I in spinach leaf discs in CO2-enriched air. Planta 228:803–812. doi: 10.1007/s00425-008-0783-4 CrossRefPubMedGoogle Scholar
  9. Joliot P, Joliot A (2002) Cyclic electron transfer in plant leaf. Proc Natl Acad Sci USA 99:10209–10214. doi: 10.1073/pnas.102306999 CrossRefPubMedGoogle Scholar
  10. Monk PMS (1998) The viologens. Physicochemical properties, synthesis and applications of the salts of 4, 4′-bipyridine. Wiley, ChichesterGoogle Scholar
  11. Ort DR, Yocum CF (1996) Electron transfer and energy transduction in photosynthesis: an overview. In: Ort DR, Yocum CF (eds) Oxygenic photosynthesis: the light reactions. Kluwer, Dordrecht, pp 1–9Google Scholar
  12. Oxborough K, Baker NR (1997) Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components—calculation of qP and Fv′/Fm′ without measuring Fo′. Photosynth Res 54:135–142. doi: 10.1023/A:1005936823310 CrossRefGoogle Scholar
  13. Renger G, Eckert H-J, Bergmann A, Bernarding J, Liu B, Napiwotzki A, Reifarth F, Eichler HJ (1995) Fluorescence and spectroscopic studies of exciton trapping and electron transfer in photosystem II of higher plants. Aust J Plant Physiol 22:167–181CrossRefGoogle Scholar
  14. Russell JH, Wallwork SC (1972) The crystal structures of the dichloride and isomorphous dibromide and diiodide of the N, N′-dimethyl-4, 4′-bipyridylium ion. Acta Crystallogr B28:1527–1533Google Scholar
  15. Schansker G, Tóth SZ, Strasser RJ (2005) Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochim Biophys Acta 1706:250–261. doi: 10.1016/j.bbabio.2004.11.006 CrossRefPubMedGoogle Scholar
  16. Schatz GH, Brock H, Holzwarth AR (1988) Kinetic and energetic model for the primary processes in photosystem II. Biophys J 54:397–405. doi: 10.1016/S0006-3495(88)82973-4 CrossRefPubMedGoogle Scholar
  17. Schreiber U (2004) Pulse–amplitude–modulation (PAM) fluorometry and saturation pulse method: an overview. In: Papageorgiou GC, Govindjee (eds) Advances in photosynthesis and respiration. Chlorophyll a fluorescence: a signature of photosynthesis, vol 19. Springer, Dordrecht, pp 279–319Google Scholar
  18. Trissl H-W, Lavergne J (1995) Fluorescence induction from photosystem II: analytical equations for the yield of photochemistry and fluorescence derived from analysis of a model including exciton-radical pair equilibrium and restricted energy transfer between photosynthetic units. Aust J Plant Physiol 22:183–193CrossRefGoogle Scholar
  19. Yruela I, Torrado E, Roncel M, Picorel R (2001) Light-induced absorption spectra of the D1–D2-cytochrome b559 complex of photosystem II: effects of methyl viologen concentration. Photosynth Res 67:199–206. doi: 10.1023/A:1010682416016 CrossRefPubMedGoogle Scholar

Copyright information

© European Biophysical Societies' Association 2009

Authors and Affiliations

  • Da-Yong Fan
    • 1
    • 2
  • Husen Jia
    • 1
  • James Barber
    • 3
  • Wah Soon Chow
    • 1
    Email author
  1. 1.Photobioenergetics Group, School of Biology, College of Medicine, Biology and EnvironmentThe Australian National UniversityCanberraAustralia
  2. 2.State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of SciencesBeijingChina
  3. 3.Division of Molecular Biosciences, Faculty of ScienceImperial CollegeLondonUK

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