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European Biophysics Journal

, Volume 38, Issue 5, pp 679–686 | Cite as

Live biospeckle laser imaging of root tissues

  • Roberto A. Braga
  • L. Dupuy
  • M. Pasqual
  • R. R. Cardoso
Original Paper

Abstract

Live imaging is now a central component for the study of plant developmental processes. Currently, most techniques are extremely constraining: they rely on the marking of specific cellular structures which generally apply to model species because they require genetic transformations. The biospeckle laser (BSL) system was evaluated as an instrument to measure biological activity in plant tissues. The system allows collecting biospeckle patterns from roots which are grown in gels. Laser illumination has been optimized to obtain the images without undesirable specular reflections from the glass tube. Data on two different plant species were obtained and the ability of three different methods to analyze the biospeckle patterns are presented. The results showed that the biospeckle could provide quantitative indicators of the molecular activity from roots which are grown in gel substrate in tissue culture. We also presented a particular experimental configuration and the optimal approach to analyze the images. This may serve as a basis to further works on live BSL in order to study root development.

Keywords

Tissue culture Biospeckle Roots Image analysis Live imaging 

Abbreviations

BSL

Biospeckle laser system

GD

Generalized differences

LASCA

Laser speckle contrast analysis

LSV

Laser speckle velocimetry

PIV

Particle image velocimetry

Notes

Acknowledgments

This study was by the Federal University of Lavras, FAPEMIG, CNPq DT, Capes and by the Scottish Executive Environment and Rural Affairs Department.

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Copyright information

© European Biophysical Societies' Association 2009

Authors and Affiliations

  • Roberto A. Braga
    • 1
  • L. Dupuy
    • 2
  • M. Pasqual
    • 1
  • R. R. Cardoso
    • 1
  1. 1.Universidade Federal de LavrasLavrasBrazil
  2. 2.Scottish Crop Research InstituteDundeeScotland, UK

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