Arrangement of ceramide [EOS] in a stratum corneum lipid model matrix: new aspects revealed by neutron diffraction studies

  • Doreen Kessner
  • Mikhail Kiselev
  • Silvia Dante
  • Thomas Hauß
  • Peter Lersch
  • Siegfried Wartewig
  • Reinhard H. H. Neubert
Original Paper

DOI: 10.1007/s00249-008-0328-6

Cite this article as:
Kessner, D., Kiselev, M., Dante, S. et al. Eur Biophys J (2008) 37: 989. doi:10.1007/s00249-008-0328-6

Abstract

The lipid matrix in stratum corneum (SC) plays a key role in the barrier function of the mammalian skin. The major lipids are ceramides (CER), cholesterol (CHOL) and free fatty acids (FFA). Especially the unique-structured ω-acylceramide CER[EOS] is regarded to be essential for skin barrier properties by inducing the formation of a long-periodicity phase of 130 Å (LPP). In the present study, the arrangement of CER[EOS], either mixed with CER[AP] and CHOL or with CER[AP], CHOL and palmitic acid (PA), inside a SC lipid model membrane has been studied for the first time by neutron diffraction. For a mixed CER[EOS]/CER[AP]/CHOL membrane in a partly dehydrated state, the internal membrane nanostructure, i.e. the neutron scattering length density profile in the direction normal to the surface, was obtained by Fourier synthesis from the experimental diffraction patterns. The membrane repeat distance is equal to that of the formerly used SC lipid model system composed of CER[AP]/CHOL/PA/ChS. By comparing both the neutron scattering length density profiles, a possible arrangement of synthetic long-chain CER[EOS] molecules inside a SC lipid model matrix is suggested. The analysis of the internal membrane nanostructure implies that one CER[EOS] molecule penetrates from one membrane layer into an adjacent layer. A 130 Å periodicity phase could not be observed under experimental conditions, either in CER/CHOL mixtures or in CER/CHOL/FFA mixture. CER[EOS] can be arranged inside a phase with a repeat unit of 45.2 Å which is predominately formed by short-chain CER[AP] with distinct polarity.

Keywords

Stratum corneum lipids Ceramide Neutron diffraction Internal membrane structure 

Abbreviations

CER[AP]

N-(α-hydroxyoctadecanoyl)-phytosphingosine

CER[EOS]

30-Linoyloxy-triacontanoic acid-[(2S,3R)-1,3-dihydroxyocta-dec-4-en-zyl]-amide

PA

Palmitic acid

CHOL

Cholesterol

ChS

Cholesterol sulphate

Copyright information

© EBSA 2008

Authors and Affiliations

  • Doreen Kessner
    • 1
  • Mikhail Kiselev
    • 2
  • Silvia Dante
    • 4
  • Thomas Hauß
    • 4
    • 5
  • Peter Lersch
    • 6
  • Siegfried Wartewig
    • 3
  • Reinhard H. H. Neubert
    • 1
  1. 1.Institute of PharmacyMartin Luther University Halle/WittenbergHalle SaaleGermany
  2. 2.Frank Laboratory of Neutron PhysicsJoint Institute for Nuclear ResearchDubnaRussia
  3. 3.Institute of Applied DermatopharmacyMartin Luther UniversityHalle SaaleGermany
  4. 4.Hahn–Meitner InstituteBerlinGermany
  5. 5.Physical Biochemistry, Department of ChemistryDarmstadt University of TechnologyDarmstadtGermany
  6. 6.Evonik Goldschmidt GmbHEssenGermany

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