European Biophysics Journal

, Volume 37, Issue 6, pp 871–877 | Cite as

A plate holder for non-destructive testing of mesophase crystallization assays

  • J. Agirre
  • A. Mechaly
  • A. Cabo-Bilbao
  • D. M. A. Guérin
Original Paper

Abstract

Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.

Keywords

Crystallization Cubic phase Membrane proteins Plate holder 

Notes

Acknowledgments

The authors are grateful to Dr. Gonzalez-Ros and Dr. Fernandez Carvajal from the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, for providing KcsA protein samples. J.A. and A.C-B.’s work was supported by Novia Salcedo Foundation grants. A.M.’s work was supported by a Ministerio de Educación y Ciencia grant. D.M.A.G.’s work was partially supported by Bizkaia Xede association. A plate holder is available upon request (20 available). Please contact Dr. Diego M. A. Guérin at diego.guerin@ehu.es.

Supplementary material

249_2008_296_MOESM1_ESM.pdf (1.7 mb)
Lipidic cubic phase dehydration (PDF 1,702 kb)

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Copyright information

© EBSA 2008

Authors and Affiliations

  • J. Agirre
    • 1
  • A. Mechaly
    • 1
  • A. Cabo-Bilbao
    • 1
    • 3
  • D. M. A. Guérin
    • 1
    • 2
  1. 1.Unidad de Biofísica (CSIC-UPV/EHU)BilbaoSpain
  2. 2.Unidad de Biofisica (CSIC-UPV/EHU)Leioa, BizkaiaSpain
  3. 3.CIC-Biogune Parque Tecnológico de Bizkaia Edificio 801 ADerioSpain

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