European Biophysics Journal

, Volume 37, Issue 6, pp 871–877 | Cite as

A plate holder for non-destructive testing of mesophase crystallization assays

  • J. Agirre
  • A. Mechaly
  • A. Cabo-Bilbao
  • D. M. A. Guérin
Original Paper


Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.


Crystallization Cubic phase Membrane proteins Plate holder 



The authors are grateful to Dr. Gonzalez-Ros and Dr. Fernandez Carvajal from the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, for providing KcsA protein samples. J.A. and A.C-B.’s work was supported by Novia Salcedo Foundation grants. A.M.’s work was supported by a Ministerio de Educación y Ciencia grant. D.M.A.G.’s work was partially supported by Bizkaia Xede association. A plate holder is available upon request (20 available). Please contact Dr. Diego M. A. Guérin at

Supplementary material

249_2008_296_MOESM1_ESM.pdf (1.7 mb)
Lipidic cubic phase dehydration (PDF 1,702 kb)


  1. Ai X, Caffrey M (2000) Biophys J 79:394–405Google Scholar
  2. Caffrey M (2000) Curr Opin Struct Biol 10:486–497CrossRefGoogle Scholar
  3. Caffrey M (2003) J Struct Biol 142:108–132CrossRefGoogle Scholar
  4. Cheng A, Hummel B, Qiu H, Caffrey M (1998) Chem Phys Lipids 95:11–21CrossRefGoogle Scholar
  5. Cherezov V, Fersi H, Caffrey M (2001) Biophys J 81:225–242CrossRefGoogle Scholar
  6. Cherezov V, Peddi A, Muthusubramaniuam L, Zheng YF, Caffrey M (2004) Acta Crystallogr D Biol Crystallogr 60:1795–1807CrossRefGoogle Scholar
  7. Cherezov V, Clogston J, Papiz MZ, Caffrey M (2006) J Mol Biol 357:1605–1618CrossRefGoogle Scholar
  8. Cribier S, Gulik A, Fellmann P, Vargas R, Devaux PF, Luzzati V (1993) J Mol Biol 229:517–525CrossRefGoogle Scholar
  9. Iwata S (2002) Methods and results in crystallization of membrane proteins. International University Line, La JollaGoogle Scholar
  10. Koynova R, Tenchov B (2001) Curr Opin Colloid Interface Sci 6:277–286CrossRefGoogle Scholar
  11. Landau E, Rosenbusch JP (1996) Proc Natl Acad Sci USA 93:14532–14535CrossRefADSGoogle Scholar
  12. Nollert P, Landau EM (1998) Biochem Soc Trans 26:709–713Google Scholar
  13. Raman P, Cherezov V, Caffrey M (2006) Cell Mol Life Sci 63:36–51CrossRefGoogle Scholar
  14. Rummel J, Hardmeyer A, Widmer C, Chiu ML, Nollert P, Locher KP, Pedruzzi I, Landau EM, Rosenbuch JP (1998) J Struct Biol 121:82–91CrossRefGoogle Scholar

Copyright information

© EBSA 2008

Authors and Affiliations

  • J. Agirre
    • 1
  • A. Mechaly
    • 1
  • A. Cabo-Bilbao
    • 1
    • 3
  • D. M. A. Guérin
    • 1
    • 2
  1. 1.Unidad de Biofísica (CSIC-UPV/EHU)BilbaoSpain
  2. 2.Unidad de Biofisica (CSIC-UPV/EHU)Leioa, BizkaiaSpain
  3. 3.CIC-Biogune Parque Tecnológico de Bizkaia Edificio 801 ADerioSpain

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