A plate holder for non-destructive testing of mesophase crystallization assays
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Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.
KeywordsCrystallization Cubic phase Membrane proteins Plate holder
The authors are grateful to Dr. Gonzalez-Ros and Dr. Fernandez Carvajal from the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, for providing KcsA protein samples. J.A. and A.C-B.’s work was supported by Novia Salcedo Foundation grants. A.M.’s work was supported by a Ministerio de Educación y Ciencia grant. D.M.A.G.’s work was partially supported by Bizkaia Xede association. A plate holder is available upon request (20 available). Please contact Dr. Diego M. A. Guérin at firstname.lastname@example.org.