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European Biophysics Journal

, Volume 37, Issue 7, pp 1241–1246 | Cite as

Role of fructose in the adaptation of plants to cold-induced oxidative stress

  • J. Bogdanović
  • M. Mojović
  • N. Milosavić
  • A. Mitrović
  • Ž. Vučinić
  • I. Spasojević
Original Paper

Abstract

This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700+). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress.

Keywords

Fructose Oxidative stress Superoxide Low temperature Photoinhibition 

Abbreviations

F6P

Fructose 6-phosphate

FBP

Fructose 1,6-bisphosphate

ROS

Reactive oxygen species

OPP

Oxidative pentose-phosphate pathway

PSI

Photosystem I

SOD

Superoxide dismutase

ABTS

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)

Notes

Acknowledgments

We are grateful to Mihajlo B. Spasić for constructive discussion. This work was supported by the Grants from the Ministry of Science of Republic of Serbia, 143016 and 143043.

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Copyright information

© EBSA 2008

Authors and Affiliations

  • J. Bogdanović
    • 1
  • M. Mojović
    • 2
  • N. Milosavić
    • 3
  • A. Mitrović
    • 1
  • Ž. Vučinić
    • 1
  • I. Spasojević
    • 1
  1. 1.Institute for Multidisciplinary ResearchBelgradeSerbia
  2. 2.Faculty for Physical ChemistryUniversity of BelgradeBelgradeSerbia
  3. 3.Department of ChemistryInstitute of Chemistry, Technology, and MetallurgyBelgradeSerbia

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