European Biophysics Journal

, Volume 36, Issue 8, pp 1015–1018

A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40

  • Mouna Marrakchi
  • Jasmina Vidic
  • Nicole Jaffrezic-Renault
  • Claude Martelet
  • Edith Pajot-Augy
Biophysics Letter

Abstract

This work shows the feasibility of an olfactory biosensor based on the immobilization of Saccharomyces cerevisiae yeast cells genetically modified to express the human olfactory receptor OR17-40 onto interdigitated microconductometric electrodes. This olfactory biosensor has been applied to the detection of its specific odorant (helional) with a high sensitivity (threshold 10−14 M). In contrast, no significant response was observed using a non-specific odorant (heptanal), which suggests a good selectivity. Thus, this work may represent a first step towards a new kind of bioelectronic noses based on whole yeast cells and allowing a real time monitoring of olfactory receptor activation.

Keywords

Olfactory biosensor Conductometric electrodes Odorant Human olfactory receptor 

Abbreviations

OR

Olfactory receptor

PBS

Sodium phosphate buffer saline

SSTR2

Somatostatin receptor 2

DMSO

Dimethylsulfoxide

Copyright information

© EBSA 2007

Authors and Affiliations

  • Mouna Marrakchi
    • 1
  • Jasmina Vidic
    • 2
  • Nicole Jaffrezic-Renault
    • 3
  • Claude Martelet
    • 1
  • Edith Pajot-Augy
    • 2
  1. 1.CEGELY, UMR-CNRS 5005Ecole Centrale de LyonEcully CedexFrance
  2. 2.Unité Neurobiologie de l’Olfaction et de la Prise Alimentaire, Equipe Récepteurs et Communication ChimiqueINRA-Université Paris-SudJouy en JosasFrance
  3. 3.Laboratoire de Sciences Analytiques, UMR CNRS 5180CNRS-Université Claude Bernard Lyon 1Villeurbanne CedexFrance

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