European Biophysics Journal

, Volume 36, Issue 8, pp 1015–1018 | Cite as

A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40

  • Mouna Marrakchi
  • Jasmina Vidic
  • Nicole Jaffrezic-Renault
  • Claude Martelet
  • Edith Pajot-Augy
Biophysics Letter


This work shows the feasibility of an olfactory biosensor based on the immobilization of Saccharomyces cerevisiae yeast cells genetically modified to express the human olfactory receptor OR17-40 onto interdigitated microconductometric electrodes. This olfactory biosensor has been applied to the detection of its specific odorant (helional) with a high sensitivity (threshold 10−14 M). In contrast, no significant response was observed using a non-specific odorant (heptanal), which suggests a good selectivity. Thus, this work may represent a first step towards a new kind of bioelectronic noses based on whole yeast cells and allowing a real time monitoring of olfactory receptor activation.


Olfactory biosensor Conductometric electrodes Odorant Human olfactory receptor 



Olfactory receptor


Sodium phosphate buffer saline


Somatostatin receptor 2




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Copyright information

© EBSA 2007

Authors and Affiliations

  • Mouna Marrakchi
    • 1
  • Jasmina Vidic
    • 2
  • Nicole Jaffrezic-Renault
    • 3
  • Claude Martelet
    • 1
  • Edith Pajot-Augy
    • 2
  1. 1.CEGELY, UMR-CNRS 5005Ecole Centrale de LyonEcully CedexFrance
  2. 2.Unité Neurobiologie de l’Olfaction et de la Prise Alimentaire, Equipe Récepteurs et Communication ChimiqueINRA-Université Paris-SudJouy en JosasFrance
  3. 3.Laboratoire de Sciences Analytiques, UMR CNRS 5180CNRS-Université Claude Bernard Lyon 1Villeurbanne CedexFrance

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