European Biophysics Journal

, Volume 36, Issue 7, pp 727–732

Atomic force microscopy images suggest aggregation mechanism in cerato-platanin

  • F. Sbrana
  • L. Bongini
  • G. Cappugi
  • D. Fanelli
  • A. Guarino
  • L. Pazzagli
  • A. Scala
  • M. Vassalli
  • C. Zoppi
  • B. Tiribilli
Original Paper

DOI: 10.1007/s00249-007-0159-x

Cite this article as:
Sbrana, F., Bongini, L., Cappugi, G. et al. Eur Biophys J (2007) 36: 727. doi:10.1007/s00249-007-0159-x

Abstract

Cerato-platanin (CP), the first member of the “cerato-platanin family”, is a moderately hydrophobic protein produced by Ceratocystis fimbriata, the causal agent of a severe plant disease called “canker stain”. The protein is localized in the cell wall of the fungus and it seems to be involved in the host-plane interaction and induces both cell necrosis and phytoalexin synthesis (one of the first plant defence-related events). Recently, it has been determined that CP, like other fungal surface protein, is able to self assemble in vitro. In this paper we characterize the aggregates of CP by Atomic Force Microscopy (AFM) images. We observe that CP tends to form early annular-shaped oligomers that seem to constitute the fundamental bricks of a hierarchical aggregation process, eventually resulting in large macrofibrillar assemblies. A simple model, based on the hypothesis that the aggregation is energetically favourable when the exposed surface is reduced, is compatible with the measured aggregates’ shape and size. The proposed model can help to understand the mechanism by which CP and many other fungal surface proteins exert their effects.

Keywords

Protein aggregation Cerato-platanin AFM 

Copyright information

© EBSA 2007

Authors and Affiliations

  • F. Sbrana
    • 1
  • L. Bongini
    • 2
  • G. Cappugi
    • 3
  • D. Fanelli
    • 1
    • 4
    • 8
  • A. Guarino
    • 5
  • L. Pazzagli
    • 3
  • A. Scala
    • 6
  • M. Vassalli
    • 1
    • 7
    • 8
  • C. Zoppi
    • 3
  • B. Tiribilli
    • 1
    • 7
  1. 1.C.S.D.C, Dip. FisicaUniversità di FirenzeSesto FiorentinoItaly
  2. 2.Dip. FisicaUniversità di FirenzeSesto FiorentinoItaly
  3. 3.Dip. di Scienze BiochimicheUniversità di FirenzeSesto FiorentinoItaly
  4. 4.Theoretical Physics, School of Physics and AstronomyUniversity of Manchester, ManchesterManchesterUK
  5. 5.Université de la Polynesie FrancaiseTahitiFrench Polynesia
  6. 6.Dip. di Biotecnologie AgrarieSezione di Patologia Vegetale, Università di FirenzeSesto FiorentinoItaly
  7. 7.ISC, CNRIstituto Sistemi ComplessiSesto FiorentinoItaly
  8. 8.I.N.F.NSezione di FirenzeSesto FiorentinoItaly

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