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European Biophysics Journal

, Volume 35, Issue 8, pp 713–719 | Cite as

Cell migration through small gaps

  • Claudia A. Brunner
  • Allen Ehrlicher
  • Bernd Kohlstrunk
  • Detlef Knebel
  • Josef A. Käs
  • Michael Goegler
Biophysics Letter

Abstract

Cell motility is a fundamental process associated with many phenomena in nature, such as immune response, wound healing, and cancer metastasis. In these processes, cells must squeeze through cell layers, and we characterize this ability to actively produce forces and simultaneously adapt their shapes. We have measured forward forces up to 15 nN that a migrating keratocyte was able to generate, in order to adjust its shape and successfully force its way under and past an obstacle. We also observed that 34 nN was capable of stalling the cell’s forward motion. Furthermore, we measured that under compression stresses up to 1,165 pN/μm2 (1,165 Pa), cell morphology, and velocity remained unchanged. Additionally, we found that keratocytes were able to compress themselves up to 80% vertically in order to squeeze through a gap as small as 500 nm.

Keywords

Indentation Depth Polystyrene Bead Cell Height Vertical Deflection Lateral Deflection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

AFM

Atomic force microscope

IRM

Interference reflection microscopy

Notes

Acknowledgments

The authors thank JPK-Instruments for their help. This work was supported by the EU project “Active Biomimetic Systems”, and the Deutsche Forschungsgemeinschaft (DFG KA 1116/3–2).

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Copyright information

© EBSA 2006

Authors and Affiliations

  • Claudia A. Brunner
    • 1
  • Allen Ehrlicher
    • 1
  • Bernd Kohlstrunk
    • 1
  • Detlef Knebel
    • 2
  • Josef A. Käs
    • 1
  • Michael Goegler
    • 1
  1. 1.Institute for Soft Matter PhysicsUniversity of LeipzigLeipzigGermany
  2. 2.JPK Instruments AGBerlinGermany

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