Micro-heterogeneity and aggregation in β2-microglobulin solutions: effects of temperature, pH, and conformational variant addition

  • Roberto Piazza
  • Matteo Pierno
  • Sara Iacopini
  • Palma Mangione
  • Gennaro Esposito
  • Vittorio Bellotti
Article
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Abstract

We show that β2-microglobulin solutions in physiological conditions contain a tiny fraction of aggregates, which can hardly be filtered out and tend to re-form spontaneously. At physiological pH the fractional amount and size distribution of the latter aggregates do not depend on temperature. Conversely, in the pH range typical of the peri-articular tissue acidosis that often occurs in hemodialysis, temperature increase leads to fast and irreversible growth of the aggregates. Quite similar, but strongly enhanced aggregation effects can be induced even in physiological conditions by adding a very small amount of ΔN6, a naturally occurring truncated isoform of β2-m known to promote fibrillogenesis.

Keywords

Fibril Dynamic Light Scattering Dynamic Light Scattering Carpal Tunnel Syndrome Hydrodynamic Radius 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

This work has been supported by grants from the Italian Ministries of Health (Grant n.08920301) and of Instruction, University, and Research (MIUR, PRIN 2003 n.2003051399 and FIRB RBNEO1S29H).

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Copyright information

© EBSA 2006

Authors and Affiliations

  • Roberto Piazza
    • 1
  • Matteo Pierno
    • 1
  • Sara Iacopini
    • 1
  • Palma Mangione
    • 2
  • Gennaro Esposito
    • 3
  • Vittorio Bellotti
    • 2
  1. 1.Dipartimento di Ingegneria NuclearePolitecnico di MilanoMilanoItaly
  2. 2.Laboratorio di Biotecnologia IRCCS Policlinico San Matteo e Dipartimento di BiochimicaUniversitá di PaviaPaviaItaly
  3. 3.Dipartimento di Scienze e Tecnologie BiomedicheUniversitá di UdineUdineItaly

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