European Biophysics Journal

, Volume 34, Issue 8, pp 1041–1048 | Cite as

Microstructural analysis of the effects of incorporation of myelin basic protein in phospholipid layers

  • L. CristofoliniEmail author
  • M. P. Fontana
  • F. Serra
  • A. Fasano
  • P. Riccio
  • O. Konovalov


We report an X-ray reflectivity study on the effects of adsorption of myelin basic protein (MBP) on Langmuir monolayers and on deposited Langmuir–Schaefer multilayers of the phospholipid dipalmitoyl phosphatidylglycerol (DPPG). We provide for the first time, direct microscopic evidence on the destructuring effects of MBP leading to plasticity of the DPPG layers supporting commonly accepted models of the stabilizing role of MBP in the myelin membrane. We also show how protein adsorption onto the layer is determined both by electrostatic and nonspecific hydrophobic interactions.


Myelin basic protein X-ray reflectivity Langmuir–Blodgett 


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Copyright information

© EBSA 2005

Authors and Affiliations

  • L. Cristofolini
    • 1
    • 5
    Email author
  • M. P. Fontana
    • 1
    • 5
  • F. Serra
    • 1
  • A. Fasano
    • 2
  • P. Riccio
    • 3
  • O. Konovalov
    • 4
  1. 1.Dipartmento di Fisica e Istituto Nazionale per la Fisica della MateriaUniversita‘ di ParmaParmaItaly
  2. 2.Dipartimento di Biochimica e Biologia MolecolareUniversita‘ di BariBariItaly
  3. 3.Dipartimento di Biologia, Difesa, Biotecnologie Agro-ForestaliUniversita‘ della Basilicata, Campus di Macchia RomanaPotenzaItaly
  4. 4.European Synchrotron Radiation FacilityGrenobleFrance
  5. 5.Istituto Nazionale per la Fisica della MateriaCentro Ricerca e Sviluppo SOFTRomeItaly

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