European Biophysics Journal

, Volume 33, Issue 2, pp 117–120

Apparatus for exposing cell membranes to rapid temperature transients

  • B. Steel
  • M. M. Bilek
  • C. G. dos Remedios
  • D. R. McKenzie
Article

Abstract

We seek to determine whether cell membranes contain sensors that trigger a downstream response to temperature excursions. To do this, we have developed a novel apparatus for exposing a cell membrane to an extremely rapid temperature excursion in the nanosecond range. Cells are plated on a gold surface that is back-heated by a pulsed laser and cooled by conduction of heat into the glass substrate and the liquid medium. Analysis using the heat diffusion equation shows that the greatest temperature rise is localized within a region tens of nanometres thick, suitable for specifically heating a cell membrane without heating the remainder of a cell. We refer to this device as a nanosecond hotplate.

Keywords

Heat diffusion equation Laser temperature jump Thermal modelling 

References

  1. Ahmadi TS, Logunov SL, El-Sayed MA (1996) Picosecond dynamics of colloidal gold nanoparticles. J Phys Chem 100:8053–8056CrossRefGoogle Scholar
  2. Chen G, Hui P (1999) Pulsed photothermal modelling of composite samples based on transmission-line theory of heat conduction. Thin Solid Films 339:58–67CrossRefGoogle Scholar
  3. Hu M, Hartland GV (2002) Heat dissipation for Au particles in aqueous solution: relaxation time versus size, J Phys Chem B 106:7029–7033Google Scholar
  4. Kaye GWC, Laby TH (1995) Tables of physical and chemical constants. Longman, Harlow, UKGoogle Scholar
  5. Lide RD (ed) (1999) CRC handbook of chemistry and physics, 79th edn. CRC Press, Boca Raton, USAGoogle Scholar
  6. Palik ED (1985) Handbook of optical constants of solids. Academic Press, Orlando, USAGoogle Scholar
  7. Radt B, Serbin J, Lange BI, Birngruber R, Huettmann G (2001) Laser generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses. Proc SPIE 4433:16–24Google Scholar
  8. Steel BC, Bilek MM, McKenzie DR, dos Remedios CG (2002) A technique for microsecond heating and cooling of a thin (submicron) biological sample. Eur Biophys J 31:378–382CrossRefPubMedGoogle Scholar
  9. Vargaftik NB, Vinogradov YK, Yargan VS (1996) Handbook of physical properties of liquids and gases. Begell House, New YorkGoogle Scholar

Copyright information

© EBSA 2004

Authors and Affiliations

  • B. Steel
    • 1
  • M. M. Bilek
    • 1
  • C. G. dos Remedios
    • 2
  • D. R. McKenzie
    • 1
  1. 1.School of PhysicsUniversity of SydneyAustralia
  2. 2.Institute of Biomedical ResearchUniversity of SydneyAustralia

Personalised recommendations