European Biophysics Journal

, Volume 31, Issue 5, pp 378–382

A technique for microsecond heating and cooling of a thin (submicron) biological sample

  • Bradley C. Steel
  • Marcela M. Bilek
  • David R. McKenzie
  • Cristobal G. dos Remedios
Article

DOI: 10.1007/s00249-002-0228-0

Cite this article as:
Steel, B.C., Bilek, M.M., McKenzie, D.R. et al. Eur Biophys J (2002) 31: 378. doi:10.1007/s00249-002-0228-0

Abstract.

Temperature excursions of short duration are useful in exploring the effects of stress on biological systems. Stress will affect the conformation of biological molecules such as proteins, which will lead to an effect on their function. The feasibility of generating such temperature excursions is demonstrated by solving the heat diffusion equation for an aqueous layer on a silicon wafer. The silicon is rapidly heated by a laser pulse and also acts as a heat sink to quench the temperature rise. An oxide layer was used to limit the maximum temperature attained by the sample. We show that exposures above a 50 °C benchmark can be confined to times less than 5 µs.

Laser temperature jump Heat diffusion equation Microwave radiation Temperature pulse 

Copyright information

© EBSA 2002

Authors and Affiliations

  • Bradley C. Steel
    • 1
  • Marcela M. Bilek
    • 1
  • David R. McKenzie
    • 1
  • Cristobal G. dos Remedios
    • 2
  1. 1.School of Physics, University of Sydney, Sydney 2006, Australia
  2. 2.Institute for Biomedical Research, Muscle Research Unit, University of Sydney, Sydney 2006, Australia

Personalised recommendations