Microbial Ecology

, Volume 33 , Issue 3 , pp 169 –179

Characterization of Viable Bacteria from Siberian Permafrost by 16S rDNA Sequencing

  • T.  Shi
  • R. H.  Reeves
  • D. A.  Gilichinsky
  • E. I.  Friedmann

DOI: 10.1007/s002489900019

Cite this article as:
Shi, T., Reeves, R., Gilichinsky, D. et al. Microb Ecol (1997) 33: 169. doi:10.1007/s002489900019

Abstract

Viable bacteria were found in permafrost core samples from the Kolyma-Indigirka lowland of northeast Siberia. The samples were obtained at different depths; the deepest was about 3 million years old. The average temperature of the permafrost is −10°C. Twenty-nine bacterial isolates were characterized by 16S rDNA sequencing and phylogenetic analysis, cell morphology, Gram staining, endospore formation, and growth at 30°C. The majority of the bacterial isolates were rod shaped and grew well at 30°C; but two of them did not grow at or above 28°C, and had optimum growth temperatures around 20°C. Thirty percent of the isolates could form endospores. Phylogenetic analysis revealed that the isolates fell into four categories: high-GC Gram-positive bacteria, β-proteobacteria, γ-proteobacteria, and low-GC Gram-positive bacteria. Most high-GC Gram-positive bacteria and β-proteobacteria, and all γ-proteobacteria, came from samples with an estimated age of 1.8–3.0 million years (Olyor suite). Most low-GC Gram-positive bacteria came from samples with an estimated age of 5,000–8,000 years (Alas suite).

Copyright information

© 1997 Springer-Verlag New York Inc.

Authors and Affiliations

  • T.  Shi
    • 1
  • R. H.  Reeves
    • 1
  • D. A.  Gilichinsky
    • 2
  • E. I.  Friedmann
    • 1
  1. 1.Department of Biological Science, Florida State University, Tallahassee, Florida 32306-2043, USAUS
  2. 2.Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, RussiaRU

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