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Microbial Ecology

, Volume 33 , Issue 3 , pp 169 –179 | Cite as

Characterization of Viable Bacteria from Siberian Permafrost by 16S rDNA Sequencing

  • T.  Shi
  • R. H.  Reeves
  • D. A.  Gilichinsky
  • E. I.  Friedmann

Abstract

Viable bacteria were found in permafrost core samples from the Kolyma-Indigirka lowland of northeast Siberia. The samples were obtained at different depths; the deepest was about 3 million years old. The average temperature of the permafrost is −10°C. Twenty-nine bacterial isolates were characterized by 16S rDNA sequencing and phylogenetic analysis, cell morphology, Gram staining, endospore formation, and growth at 30°C. The majority of the bacterial isolates were rod shaped and grew well at 30°C; but two of them did not grow at or above 28°C, and had optimum growth temperatures around 20°C. Thirty percent of the isolates could form endospores. Phylogenetic analysis revealed that the isolates fell into four categories: high-GC Gram-positive bacteria, β-proteobacteria, γ-proteobacteria, and low-GC Gram-positive bacteria. Most high-GC Gram-positive bacteria and β-proteobacteria, and all γ-proteobacteria, came from samples with an estimated age of 1.8–3.0 million years (Olyor suite). Most low-GC Gram-positive bacteria came from samples with an estimated age of 5,000–8,000 years (Alas suite).

Keywords

Phylogenetic Analysis Growth Temperature Cell Morphology Core Sample Bacterial Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • T.  Shi
    • 1
  • R. H.  Reeves
    • 1
  • D. A.  Gilichinsky
    • 2
  • E. I.  Friedmann
    • 1
  1. 1.Department of Biological Science, Florida State University, Tallahassee, Florida 32306-2043, USAUS
  2. 2.Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, RussiaRU

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