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Arachis hypogaea L. from Acid Soils of Nanyang (China) Is Frequently Associated with Bradyrhizobium guangdongense and Occasionally with Bradyrhizobium ottawaense or Three Bradyrhizobium Genospecies

  • Soil Microbiology
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Abstract

Henan Province is a major area of peanut production in China but the rhizobia nodulating the crop in this region have not been described. A collection of 217 strains of peanut rhizobia was obtained from six field sites across four soil types in Henan Province, North China, by using peanut as a trap host under glasshouse conditions. The 217 strains separated into 8 distinct types on PCR–RFLP analysis of their IGS sequences. Phylogenetic analysis of the 16S rRNA, recA, atpD, and glnII genes of 11 representative strains of the 8 IGS types identified Bradyrhizobium guangdongense, B. ottawaense and three novel Bradyrhizobium genospecies. Bradyrhizobium guangdongense was dominant, accounting for 75.0% of the total isolates across the field sites while B. ottawaense covered 5.1% and the three novel Bradyrhizobium genospecies 4.1 to 8.8% of the total. The symbiosis-related nodA and nifH gene sequences were not congruent with the core genes on phylogenetic analysis and separated into three groups, two of which were similar to sequences of Bradyrhizobium spp. isolated from peanut in south-east China and the third identical to that of B. yuanmingense isolated from Lespedeza cuneata in northern China. A canonical correlation analysis between the distribution of IGS genotypes and soil physicochemical characteristics and climatic factors indicated that the occurrence of IGS types/species was mainly associated with soil pH and available phosphorus.

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Funding

This work was financed by the Project for Extramural Scientists of State Key Laboratory of Agrobiotechnology (Project No. 2021SKLAB6-8) from JJ Zhang and project of Sabbatical Year SIP20200726 authorized by IPN, Mexico from ET Wang.

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Authors and Affiliations

  1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Henan Province, Zhengzhou, 450000, People’s Republic of China

    Junjie Zhang, Shanshan Peng & Shuo Li

  2. Collaborative Innovation Center for Food Production and Safety of Henan Province, Henan Province, Zhengzhou, 450002, People’s Republic of China

    Junjie Zhang

  3. Nanyang Academy of Agricultural Sciences, Henan Province, Nanyang, 473000, People’s Republic of China

    Jiangchun Song

  4. LSTM, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France

    Brigitte Brunel

  5. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, México, D. F., México

    Entao Wang

  6. The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK

    Euan K. James

  7. State Key Laboratory of Agrobiotechnology, Beijing, 100193, People’s Republic of China

    Wenfeng Chen

  8. College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing, 100193, People’s Republic of China

    Wenfeng Chen

  9. Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647, New Zealand

    Mitchell Andrews

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  1. Junjie Zhang
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Zhang, J., Peng, S., Li, S. et al. Arachis hypogaea L. from Acid Soils of Nanyang (China) Is Frequently Associated with Bradyrhizobium guangdongense and Occasionally with Bradyrhizobium ottawaense or Three Bradyrhizobium Genospecies. Microb Ecol 84, 556–564 (2022). https://doi.org/10.1007/s00248-021-01852-2

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