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Natural Communities of Carotenogenic Chlorophyte Haematococcus lacustris and Bacteria from the White Sea Coastal Rock Ponds

  • Anna Kublanovskaya
  • Alexei Solovchenko
  • Tatyana Fedorenko
  • Konstantin ChekanovEmail author
  • Elena Lobakova
Microbiology of Aquatic Systems
  • 55 Downloads

Abstract

Haematococcus lacustris is a biotechnologically important green unicellular alga producing widely used keta-karotenoid astaxanthin. In natural habitats, it exists in the form of algal-bacterial community, and under laboratory conditions, it is also accompanied by bacteria. The issue of the bacterial composition of industrial algal cultures is widely recognized as important. However, there is a dearth of information about bacterial composition of H. lacustris communities. In current work, we analyze the composition of natural H. lacustris communities from the White Sea coastal temporal rock ponds. For the first time, a 16S rRNA gene-based metagenome of natural H. lacustris bacterial communities has been generated. Main results of its analysis are as follow. Bacterial families Comamonadaceae, Cytophagaceae, Xanthomonadaceae, Acetobacteraceae, Rhodobacteraceae, and Rhodocyclaceae were observed in all studied H. lacustris natural communities. They also contained genera Hydrogenophaga and Cytophaga. Bacteria from the Hydrogenophaga genus were present in H. lacustris cultures after their isolation under the conditions of laboratory cultivation. Similar to other planktonic microalgae, H. lacustris forms a phycosphere around the cells. In this zone, bacteria attached to the algal surface. The contact between H. lacustris and bacteria is maintained even after sample drying. The study provides information about possible members of H. lacustris core microbiome, which can be presented in the industrial and laboratory cultures of the microalga.

Keywords

Haematococcus lacustris Algal-bacterial community Microbial communities Metagenome Carotenogenic algae 

Abbreviations

ACE

Abundance-based coverage

NACE

Abundance-based coverage estimator

DOC

Dissolved organic carbon

eDNA

Environmental DNA

EPS

Exopolysaccharides

NChao − 1

Non-parametric Chao-1 estimator

N

Observed number of taxa

OUT

Operation taxonomic unit

SEM

Scanning electron microscopy

H

Shannon entropy index

d

Simpson reverse index

Notes

Acknowledgments

Microscopic studies were conducted using equipment of the Center of Microscopy of White Sea Biological Station of Moscow State University. A part of the work was also supported by Council for grants of the President of the Russian Federation.

Funding Information

This study funded by the Russian Foundation for Basic Research (grant no. 18-29-25050). The publication was prepared with partial support of the RUDN University Program 5-100. A part of the work was also supported by Council for grants of the President of the Russian Federation.

Supplementary material

248_2019_1437_MOESM1_ESM.pdf (130 kb)
ESM 1 (PDF 129 kb)

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Copyright information

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Authors and Affiliations

  1. 1.Department of Bioengineering, Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Peoples Friendship University of Russia (RUDN University)MoscowRussia
  3. 3.Centre for Humanities Research and TechnologyNational Research Nuclear University MEPhiMoscowRussia

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