Diversity and Distribution of Bacteria Producing Known Secondary Metabolites
There is an increasing interest in the utilisation of marine bioactive compounds as novel biopharmaceuticals and agrichemicals; however, little is known about the environmental distribution for many of these molecules. Here, we aimed to elucidate the environmental distribution and to detect the biosynthetic gene clusters in environmental samples of four bioactive compounds, namely violacein, tropodithietic acid (TDA), tambjamine and the antibacterial protein AlpP. Our database analyses revealed high bacterial diversity for AlpP and violacein producers, while TDA-producing bacteria were mostly associated with marine surfaces and all belonged to the roseobacter group. In contrast, the tambjamine cluster was only found in the genomes of two Pseudoalteromonas species and in one terrestrial species belonging to the Cupriavidus genus. Using a PCR-based screen of different marine samples, we detected TDA and violacein genes associated with the microbiome of Ulva and Protohyale niger and tambjamine genes associated with Nodilittorina unifasciata; however, alpP was not detected. These results highlight the variable distribution of the genes encoding these four bioactive compounds, including their detection from the surface of multiple marine eukaryotic hosts. Determining the natural distribution of these gene clusters will help to understand the ecological importance of these metabolites and the bacteria that produce them.
KeywordsMarine bioactive compounds Surface-associated bacteria Antibiotic-producing bacteria (APB) Violacein Tropodithietic acid (TDA) Tambjamine AlpP
The authors thank Torsten Thomas and members of the CMB group for feedback on the manuscript.
This work was supported by an Australian Research Council (ARC) Future Fellowship awarded to Suhelen Egan (grant number: FT130100828).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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