Microbial Ecology

, Volume 78, Issue 4, pp 792–803 | Cite as

Influences of Iron Compounds on Microbial Diversity and Improvements in Organic C, N, and P Removal Performances in Constructed Wetlands

  • Zhimiao Zhao
  • Xiao Zhang
  • Mengqi Cheng
  • Xinshan SongEmail author
  • Yinjiang Zhang
  • Xiangmei Zhong
Microbiology of Aquatic Systems


The effects of various combinations of iron compounds on the contaminant removal performance in constructed wetlands (CWs) were explored under various initial iron concentrations, contaminant concentrations, different hydraulic retention time (HRT), and different temperatures. The Combo 6 (nanoscale zero-valent iron combined with Fe3+) in CW treatments showed the highest pollutant removal performance under the conditions of C2 initial iron dosage concentration (total iron 0.2 mM) and I2 initial contaminant concentration (COD:TN:TP = 60 mg/L:60 mg/L:1 mg/L) in influent after 72-h HRT. These results were directly verified by two different microbial tests (Biolog test and high-throughput pyrosequencing) and microbial community analysis (principal component analysis of community-level physiological profile, biodiversity index, cluster tree, relative abundance at order of taxonomy level). Specific bacteria related to significant improvements in contaminant removal were domesticated by various combinations of iron compounds. Iron dosage was advised as a green, new, and effective option for wastewater treatment.

Graphical Abstract



Combinations of iron compounds Multiple contaminants Oxidation-reduction Microbial community optimization 

Supplementary material

248_2019_1379_MOESM1_ESM.docx (2 mb)
ESM 1 (DOCX 2000 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhimiao Zhao
    • 1
    • 2
  • Xiao Zhang
    • 1
    • 2
  • Mengqi Cheng
    • 1
    • 2
  • Xinshan Song
    • 3
    Email author
  • Yinjiang Zhang
    • 1
    • 2
  • Xiangmei Zhong
    • 1
    • 2
  1. 1.College of Marine Ecology and EnvironmentShanghai Ocean UniversityShanghaiChina
  2. 2.Engineering Research Center for Water Environment Ecology in ShanghaiShanghaiChina
  3. 3.State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, College of Environmental Science and EngineeringDonghua UniversityShanghaiChina

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