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Bacterial Community Composition and Diversity Respond to Nutrient Amendment but Not Warming in a Maritime Antarctic Soil

  • Kevin K. NewshamEmail author
  • Binu M. Tripathi
  • Ke Dong
  • Naomichi Yamamoto
  • Jonathan M. Adams
  • David W. Hopkins
Soil Microbiology
  • 39 Downloads

Abstract

A resumption of climate warming in maritime Antarctica, arising from continued greenhouse gas emissions to the atmosphere, is predicted to lead to further expansions of plant populations across the region, with consequent increases in nutrient inputs to soils. Here, we test the main and interactive effects of warming, applied with open top chambers (OTCs), and nutrient amendment with tryptic soy broth (TSB), an artificial growth substrate, on bacterial community composition and diversity using Illumina sequencing of 16S rRNA genes in soil from a field experiment in the southern maritime Antarctic. Substantial effects of TSB application on bacterial communities were identified after 49 months, including reduced diversity, altered phylogenetic community assembly processes, increased Proteobacteria-to-Acidobacteria ratios and significant divergence in community composition, notably increases in the relative abundances of the gram-positive genera Arthrobacter, Paeniglutamicibacter and Planococcus. Contrary to previous observations from other maritime Antarctic field warming experiments, we recorded no effects of warming with OTCs, or interactive effects of OTCs and TSB application, on bacterial community composition or diversity. Based on these findings, we conclude that further warming of the maritime Antarctic is unlikely to influence soil bacterial community composition or diversity directly, but that increased nutrient inputs arising from enhanced plant growth across the region may affect the composition of soil bacterial communities, with possible effects on ecosystem productivity.

Keywords

Antarctica Bacterial community composition Climate warming Gram-positive and gram-negative bacteria Nutrient inputs Proteobacteria-to-Acidobacteria ratio 

Notes

Acknowledgements

Logistical support was provided by the British Antarctic Survey’s Operations Unit, with Air Unit pilots Alan Meredith, Steve King, Doug Pearson and Ian Potten providing access to Mars Oasis. Adam Clark, Dickie Hall, Sharon Duggan and Paul Dennis provided valuable support. Two anonymous reviewers provided helpful comments. All are gratefully acknowledged.

Funding Information

This research was funded by the British Antarctic Survey’s Long-Term Monitoring and Survey programme, the NERC Antarctic Funding Initiative (grant number NE/D00893X/1) and a National Research Foundation of Korea Grant from the Korean Government (grant number 2018R1C1B6007755).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

248_2019_1373_MOESM1_ESM.pdf (247 kb)
ESM 1 (PDF 246 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.NERC British Antarctic SurveyCambridgeUK
  2. 2.Korea Polar Research InstituteIncheonRepublic of Korea
  3. 3.Department of Life Science, College of Natural SciencesKyonggi UniversitySuwonRepublic of Korea
  4. 4.Department of Environmental Health SciencesSeoul National UniversitySeoulRepublic of Korea
  5. 5.Institute of Soil ScienceChinese Academy of SciencesNanjingChina
  6. 6.Scotland’s Rural CollegeEdinburghUK

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