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Time-Dependent Hormetic Response of Soil Alkaline Phosphatase Induced by Cd and the Association with Bacterial Community Composition

  • Jiangang HanEmail author
  • Shengyan Wang
  • Diwu Fan
  • Yanhui Guo
  • Chenglei Liu
  • Yongli Zhu
Soil Microbiology
  • 71 Downloads

Abstract

Hormetic dose-response that involved Cd in soils is increasingly paid attentions for risk assessment of Cd toxicity, but insufficient studies were conducted to define the temporary modification of soil enzyme and the potential microbial responses. The present study chooses soil alkaline phosphatase (ALP) as endpoint to uncover the time-dependent hormetic responses to low doses of Cd and its association with bacterial community composition. The results showed that addition of 0.01–3.0 mg kg−1 Cd significantly increased ALP’s activities with maximum stimulatory magnitude of 11.4–27.2%, indicating a typical hormesis. The response started at 12 h after Cd addition and maintained about 24 h. This demonstrated that the hormetic response is time-dependent and transient. Changes of soil bacterial community composition showed that, at 6 h, relative abundances (RAs) of Proteobacteria and Firmicutes at phylum and Pontibacter, Bacillaceae-Bacillus, Bacillaceae1-Bacillus, and Paenisporosarcina at genus significantly correlated with ALP’s activities at 12–36 h (P < 0.05). This suggests that soil bacteria likely showed an earlier response to Cd and potentially contributes to the subsequent soil enzyme’s hormesis. In addition, it was found that Gram-negative bacteria other than Gram-positive bacteria are prone to exhibiting a hormetic response under Cd stress. Our findings provide much insight into ecotoxicological risk assessment for soil Cd pollution.

Keywords

Hormesis Cadmium Alkaline phosphatase Bacterial community composition 

Notes

Acknowledgements

We thank all the supporters of this article for their constructive comments.

Funding Information

This study was supported by the financial support of National Natural Science Foundation of China (No. 41375149, No. 41471191), Qing Lan Project of Jiangsu Province (Qinglan2016-15), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jiangang Han
    • 1
    • 2
    Email author
  • Shengyan Wang
    • 1
  • Diwu Fan
    • 1
  • Yanhui Guo
    • 1
  • Chenglei Liu
    • 1
  • Yongli Zhu
    • 1
  1. 1.College of Biology and the EnvironmentNanjing Forestry UniversityNanjingPeople’s Republic of China
  2. 2.Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu ProvinceNanjing Forestry UniversityNanjingPeople’s Republic of China

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