Latitudinal and Vertical Variation of Synechococcus Assemblage Composition Along 170° W Transect From the South Pacific to the Arctic Ocean
Synechococcus is one of the most widely distributed and abundant picocyanobacteria in the global oceans. Although latitudinal variation of Synechococcus assemblage in marine surface waters has been observed, few studies compared Synechococcus assemblage composition in surface and subsurface waters at the basin scale. Here, we report marine Synechococcus diversity in the surface and deep chlorophyll maximum (DCM) layers along 170° W from the South Pacific to the Arctic Ocean in summer. Along the transect, spatial niche partitioning of Synechococcus lineages in the surface waters was clearly observed. Species richness of surface Synechococcus assemblage was positively correlated with water temperature. Clade CRD1 was dominant in the areas (15° S–10° N and 35–40° N) associated with upwelling, and there were 3 different subclades with distinct distribution. CRD1-A was restricted in the North Equatorial Current (5–10° N), CRD1-B dominated in the equatorial upwelling region (15° S–0.17° N), and CRD1-C was only distributed in the North Pacific Current (35–40° N). Similarities between the Synechococcus assemblages in the surface and DCM layers were high at the upwelling regions and areas where the mixed layer was deep, while low in the Subtropical Gyres with strong stratification. Clade I, CRD1-B, and CRD1-C were major Synechococcus lineages in the DCM layer. In particular, clade I, which is composed of 7 subclades with distinct thermal niches, was widely distributed in the DCM layer. Overall, our results provide new insights into not only the latitudinal distribution of Synechococcus assemblages, but also their vertical variation in the central Pacific.
KeywordsHorizontal and vertical variations Synechococcus assemblage richness CRD1 Clade I Central Pacific Ocean
We wish to thank the captain, officers, and crew of the R/V Hakuho Maru for their tremendous assistance during the cruises. We are grateful to Drs. Koji Sugie and Jun Nishioka for the field sampling and nutrient analysis, respectively. This study was conducted within the framework of the JST-CREST program “Establishment of core technology for the preservation of marine diversity and ecosystems”.
The JST-CREST Program (JPMJCR11A5), JSPS Grant-in-Aid for Scientific Research on Innovative Areas (#24121004), Research Grant Council of Hong Kong (16128416 and 16101917), and the National Key Scientific Research Project (2015CB954003) sponsored by the Ministry of Science and Technology of the PRC partly funded this study. This work was also supported by CAS Pioneer Hundred Talents Program and the South China Sea Institute of Oceanography, CAS for the project “Different niches of Synechococcus ecotypes (50603-64)”.
- 1.Flombaum P, Gallegos JL, Gordillo RA, Rincon J, Zabala LL, Jiao N, Karl DM, Li WK, Lomas MW, Veneziano D, Vera CS, Vrugt JA, Martiny AC (2013) Present and future global distributions of the marine cyanobacteria. Prochlorococcus and Synechococcus. Proc Natl Acad Sci 110:9824–9829CrossRefGoogle Scholar
- 2.Partensky F, Blanchot J, Vaulot D (1999) Differential distribution and ecology of Prochlorococcus and Synechococcus in oceanic waters: a review. Bulletin-institut oceanographique monaco-numero special 457–476Google Scholar
- 7.Farrant GK, Dore H, Cornejo-Castillo FM, Partensky F, Ratin M, Ostrowski M, Pitt FD, Wincker P, Scanlan DJ, Iudicone D, Acinas SG, Garczarek L (2016) Delineating ecologically significant taxonomic units from global patterns of marine picocyanobacteria. Proc Natl Acad Sci 113:E3365–E3374CrossRefGoogle Scholar
- 19.Zwirglmaier K, Jardillier L, Ostrowski M, Mazard S, Garczarek L, Vaulot D, Not F, Massana R, Ulloa O, Scanlan DJ (2008) Global phylogeography of marine Synechococcus and Prochlorococcus reveals a distinct partitioning of lineages among oceanic biomes. Environ Microbiol 10:147–161PubMedGoogle Scholar
- 20.Ahlgren NA, Noble A, Patton AP, Roache-Johnson K, Jackson L, Robinson D, McKay C, Moore LR, Saito MA, Rocap G (2014) The unique trace metal and mixed layer conditions of the Costa Rica upwelling dome support a distinct and dense community of Synechococcus. Limnol Oceanogr 59:2166–2184CrossRefGoogle Scholar
- 29.Lavin P, Gomez P, Gonzalez B, Ulloa O (2008) Diversity of the marine picocyanobacteria Prochlorococcus and Synechococcus assessed by terminal restriction fragment length polymorphisms of 16S-23S rRNA internal transcribed spacer sequences. Rev Chil Hist Nat 81Google Scholar
- 40.Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541CrossRefGoogle Scholar
- 41.Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. vol. 41. Information Retrieval Ltd., London, pp 95–98Google Scholar
- 43.Wei T, Simko V, Levy M, Xie Y, Jin Y, Zemla J (2017) Package ‘corrplot’. Statistician 56:316–324Google Scholar
- 44.Oksanen J, Kindt R, Legendre P, O’Hara B, Stevens MHH, Oksanen MJ, Suggests M (2007) The vegan package. Community ecology package 10:631–637Google Scholar
- 48.Aumont O, Bopp L (2006) Globalizing results from ocean in situ iron fertilization studies. Glob Biogeochem Cycles 20Google Scholar
- 51.Grébert T, Doré H, Partensky F, Farrant GK, Boss ES, Picheral M, Guidi L, Pesant S, Scanlan DJ, Wincker P (2018) Light color acclimation is a key process in the global ocean distribution of Synechococcus cyanobacteria. Proc Natl Acad Sci 201717069Google Scholar