Microbiome Diversity in Cotton Rhizosphere Under Normal and Drought Conditions
Climate change contributes to drought stress and subsequently affects crop growth, development, and yield. The microbial community, such as fungi and bacteria in the rhizosphere, is of special importance to plant productivity. In this study, soil collected from a cotton research field was used to grow cotton plants (Gossypium hirsutum cv. Jin668) under controlled environment conditions. Drought stress was applied at flowering stage, while control plants were regularly watered. At the same time, the soil without plants was also subjected to drought, while control pots were regularly watered. The soil was collected in sterilized tubes and microbial DNA was isolated and high-throughput sequencing of 16S rRNA genes was carried out. The alpha diversity of bacteria community significantly increased in the soil with cotton plants compared to the soil without cotton plants. Taxonomic analysis revealed that the bacterial community structure of the cotton rhizosphere predominantly consisted of the phyla Proteobacteria (31.7%), Actinobacteria (29.6%), Gemmatimonadetes (9.8%), Chloroflexi (9%), Cyanobacteria (5.6%), and Acidobacteria. In the drought-treated rhizosphere, Chloroflexi and Gemmatimonadetes were the dominant phyla. This study reveals that the cotton rhizosphere has a rich pool of bacterial communities even under drought stress, and which may improve drought tolerance in plants. These data will underpin future improvement of drought tolerance of cotton via the soil microbial community.
KeywordsCotton Drought Microbial diversity Plant-microbe interactions Rhizosphere
Xiyan Yang designed the project; Abid Ullah and Adnan Akbar conducted the experiments and wrote the paper; Qingqing Luo, Hakim, and Muhammad Shaban helped Abid Ullah in the experiments; Aamir Hamid Khan helped Abid Ullah in writing the paper; and Xiyan Yang critically reviewed the paper.
Funding was provided by the National Key Project of Research and Development Plan (2016YFD0101006) and National R&D Project of Transgenic Crops of Ministry of Science and Technology of China (2016ZX08005-004-002). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Compliance with Ethical Standards
The authors declare that they have no competing interests.
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