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Co-occurrence Networks Among Bacteria and Microbial Eukaryotes of Lake Baikal During a Spring Phytoplankton Bloom

  • Ivan S. Mikhailov
  • Yulia R. Zakharova
  • Yuri S. Bukin
  • Yuri P. Galachyants
  • Darya P. Petrova
  • Maria V. Sakirko
  • Yelena V. Likhoshway
Environmental Microbiology

Abstract

The pelagic zone of Lake Baikal is an ecological niche where phytoplankton bloom causes increasing microbial abundance in spring which plays a key role in carbon turnover in the freshwater lake. Co-occurrence patterns revealed among different microbes can be applied to predict interactions between the microbes and environmental conditions in the ecosystem. We used 454 pyrosequencing of 16S rRNA and 18S rRNA genes to study bacterial and microbial eukaryotic communities and their co-occurrence patterns at the pelagic zone of Lake Baikal during a spring phytoplankton bloom. We found that microbes within one domain mostly correlated positively with each other and are highly interconnected. The highly connected taxa in co-occurrence networks were operational taxonomic units (OTUs) of Actinobacteria, Bacteroidetes, Alphaproteobacteria, and autotrophic and unclassified Eukaryota which might be analogous to microbial keystone taxa. Constrained correspondence analysis revealed the relationships of bacterial and microbial eukaryotic communities with geographical location.

Keywords

Co-occurrence network Bacteria Microbial eukaryotes Community Lake Baikal Pyrosequencing 

Notes

Acknowledgements

We thank R.Yu. Gnatovsky and V.V. Blinov from the hydrology and hydrophysics laboratory of Limnological Institute of SB RAS for collecting water samples and providing temperature data.

Funding information

This work was supported by the Federal Agency of Scientific Organizations (FASO) of Russia under the subject No. 0345-2016-0005 (pyrosequencing results) and the subject No. 0345-2016-0001 (metagenomic and statistical analyzes).

Supplementary material

248_2018_1212_MOESM1_ESM.doc (4.8 mb)
ESM 1 (DOC 4902 kb)

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Authors and Affiliations

  1. 1.Limnological InstituteSiberian Branch of the Russian Academy of SciencesIrkutskRussia

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