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Microbial Ecology

, Volume 76, Issue 4, pp 1021–1029 | Cite as

The Role of Iron Competition in the Antagonistic Action of the Rice Endophyte Streptomyces sporocinereus OsiSh-2 Against the Pathogen Magnaporthe oryzae

  • Jiarui Zeng
  • Ting Xu
  • Lidan Cao
  • Chunyi Tong
  • Xuan Zhang
  • Dingyi Luo
  • Shuping Han
  • Pei Pang
  • Weibin Fu
  • Jindong Yan
  • Xuanming Liu
  • Yonghua ZhuEmail author
Plant Microbe Interactions

Abstract

Rice blast caused by Magnaporthe oryzae severely impacts global rice yield stability. The rice endophyte Streptomyces sporocinereus OsiSh-2, with strong antagonistic activity towards M. oryzae, has been reported in our previous study. To decipher the model of the antagonistic action of OsiSh-2 towards M. oryzae, we compared the iron-capturing abilities of these two strains. The cultivation of OsiSh-2 and a M. oryzae strain under iron-rich and iron-starved conditions showed that M. oryzae depended more on iron supplementation for growth and development than did OsiSh-2. Genomic analysis of the S. sporocinereus and M. oryzae species strains revealed that they might possess different iron acquisition strategies. The actinobacterium OsiSh-2 is likely to favor siderophore utilization compared to the fungus M. oryzae. In addition, protein annotations found that OsiSh-2 contains the highest number of the siderophore biosynthetic gene clusters among the 13 endophytic actinomycete strains and 13 antifungal actinomycete strains that we compared, indicating the prominent siderophore production potential of OsiSh-2. Additionally, we verified that OsiSh-2 could excrete considerably more siderophores than Guy11 under iron-restricted conditions and displayed greater Fe3+-reducing activity during iron-supplemental conditions. Measurements of the iron mobilization between the antagonistic OsiSh-2 and Guy11 showed that the iron concentration is higher around OsiSh-2 than around Guy11. In addition, adding iron near OsiSh-2 could decrease the antagonism of OsiSh-2 towards Guy11. Our study revealed that the antagonistic capacity displayed by OsiSh-2 towards M. oryzae was related to the competition for iron. The highly efficient iron acquisition system of OsiSh-2 may offer valuable insight for the biocontrol of rice blast.

Keywords

Endophyte Streptomyces sporocinereus OsiSh-2 Magnaporthe oryzae Siderophore Iron competition 

Notes

Funding Information

This work is financially supported by the National Natural Science Foundation of China (31672093).

Supplementary material

248_2018_1189_MOESM1_ESM.docx (14 kb)
Table S1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jiarui Zeng
    • 1
  • Ting Xu
    • 1
  • Lidan Cao
    • 1
  • Chunyi Tong
    • 1
  • Xuan Zhang
    • 1
  • Dingyi Luo
    • 1
  • Shuping Han
    • 1
  • Pei Pang
    • 2
  • Weibin Fu
    • 1
  • Jindong Yan
    • 1
  • Xuanming Liu
    • 1
  • Yonghua Zhu
    • 1
    Email author
  1. 1.Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of BiologyHunan UniversityChangshaPeople’s Republic of China
  2. 2.College of Information Science and EngineeringHunan UniversityChangshaPeople’s Republic of China

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