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Metagenomic Analysis of Zinc Surface–Associated Marine Biofilms

  • Wei Ding
  • Weipeng Zhang
  • Nabeel Mannalamkunnath Alikunhi
  • Zenon Batang
  • Bite Pei
  • Ruojun Wang
  • Lianguo Chen
  • Abdulaziz Al-Suwailem
  • Pei-Yuan QianEmail author
Environmental Microbiology

Abstract

Biofilms are a significant source of marine biofouling. Marine biofilm communities are established when microorganisms adhere to immersed surfaces. Despite the microbe-inhibiting effect of zinc surfaces, microbes can still attach to the surface and form biofilms. However, the diversity of biofilm-forming microbes that can attach to zinc surfaces and their common functional features remain elusive. Here, by analyzing 9,000,000 16S rRNA gene amplicon sequences and 270 Gb of metagenomic data, we comprehensively explored the taxa and functions related to biofilm formation in subtidal zones of the Red Sea. A clear difference was observed between the biofilm and adjacent seawater microbial communities in terms of the taxonomic structure at phylum and genus levels, and a huge number of genera were only present in the biofilms. Saturated alpha-diversity curves suggested the existence of more than 14,000 operational taxonomic units in one biofilm sample, which is much higher than previous estimates. Remarkably, the biofilms contained abundant and diverse transposase genes, which were localized along microbial chromosomal segments and co-existed with genes related to metal ion transport and resistance. Genomic analyses of two cyanobacterial strains that were abundant in the biofilms revealed a variety of metal ion transporters and transposases. Our analyses revealed the high diversity of biofilm-forming microbes that can attach to zinc surfaces and the ubiquitous role of transposase genes in microbial adaptation to toxic metal surfaces.

Keywords

Marine biofilm Zinc panel Transposase Metagenome 

Notes

Acknowledgments

This study was supported by a research grant from China Ocean Mineral Resource Research and Development Association (COMRRDA17/Sc01) and an award from the King Abdullah University of Science and Technology to P.Y. Qian. The authors are grateful to Ms. Alice Cheung for English editing.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

248_2018_1313_MOESM1_ESM.docx (2 mb)
ESM 1 (DOCX 2087 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Wei Ding
    • 1
  • Weipeng Zhang
    • 2
  • Nabeel Mannalamkunnath Alikunhi
    • 3
  • Zenon Batang
    • 3
  • Bite Pei
    • 2
  • Ruojun Wang
    • 2
  • Lianguo Chen
    • 1
  • Abdulaziz Al-Suwailem
    • 3
  • Pei-Yuan Qian
    • 1
    • 2
    Email author
  1. 1.Division of Life ScienceHong Kong University of Science and TechnologyHong KongChina
  2. 2.Department of Ocean ScienceHong Kong University of Science and TechnologyHong KongChina
  3. 3.King Abdullah University of Science and TechnologyThuwalSaudi Arabia

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