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Microbial Ecology

, Volume 76, Issue 1, pp 215–225 | Cite as

Distinctive Soil Archaeal Communities in Different Variants of Tropical Equatorial Forest

  • Dorsaf Kerfahi
  • Binu M. Tripathi
  • J. W. Ferry Slik
  • Rahayu S. Sukri
  • Salwana Jaafar
  • Jonathan M. AdamsEmail author
Soil Microbiology

Abstract

Little is known of how soil archaeal community composition and diversity differ between local variants of tropical rainforests. We hypothesized that (1) as with plants, animals, fungi, and bacteria, the soil archaeal community would differ between different variants of tropical forest; (2) that spatially rarer forest variants would have a less diverse archaeal community than common ones; (3) that a history of forest disturbance would decrease archaeal alpha- and beta-diversity; and (4) that archaeal distributions within the forest would be governed more by deterministic than stochastic factors. We sampled soil across several different forest types within Brunei, Northwest Borneo. Soil DNA was extracted, and the 16S rRNA gene of archaea was sequenced using Illumina MiSeq. We found that (1) as hypothesized, there are distinct archaeal communities for each forest type, and community composition significantly correlates with soil parameters including pH, organic matter, and available phosphorous. (2) As hypothesized, the “rare” white sand forest variants kerangas and inland heath had lower archaeal diversity. A nestedness analysis showed that archaeal community in inland heath and kerangas was mainly a less diverse subset of that in dipterocarp forests. However, primary dipterocarp forest had the lowest beta-diversity among the other tropical forest types. (3) Also, as predicted, forest disturbance resulted in lower archaeal alpha-diversity—but increased beta-diversity in contrast with our predictions. (4) Contrary to our predictions, the BetaNTI of the various primary forest types indicated community assembly was mainly stochastic. The possible effects of these habitat and disturbance-related effects on N cycling should be investigated.

Keywords

Archaeal composition Archaeal diversity Kerangas, Tropical forests Inland heath forest White sand forest 

Notes

Acknowledgments

This work was supported by a grant from the National Research Foundation (NRF) funded by the Korean Government, Ministry of Education, Science and Technology (MEST) (NRF-0409-20150076). We thank the Brunei Forestry Department and the Biodiversity Research and Innovation Centre for entry and export permits, respectively, and the Universiti Brunei Darussalam for permission to conduct research.

Supplementary material

248_2017_1118_MOESM1_ESM.docx (431 kb)
ESM 1 (DOCX 430 kb)

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© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Dorsaf Kerfahi
    • 1
    • 2
  • Binu M. Tripathi
    • 3
  • J. W. Ferry Slik
    • 4
  • Rahayu S. Sukri
    • 4
  • Salwana Jaafar
    • 4
  • Jonathan M. Adams
    • 5
    Email author
  1. 1.Department of Biological SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Biological OceanographyLeibniz Institute for Baltic Sea Research Warnemünde (IOW)RostockGermany
  3. 3.Korea Polar Research InstituteIncheonRepublic of Korea
  4. 4.Faculty of ScienceUniversiti Brunei DarussalamGadongBrunei Darussalam
  5. 5.Division of Agrifood and EnvironmentCranfield UniversityCranfieldUK

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