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Bacterial Signatures of “Red-Operculum” Disease in the Gut of Crucian Carp (Carassius auratus)

Abstract

Fish gut microbiota play important roles in fish immunity, nutrition, and the adaptation to environmental changes. To date, few studies have focused on the interactions among environmental factors, fish diseases, and gut microbiota compositions. We compared the gut bacterial communities of healthy crucian carps (Carassius auratus) with those of individuals affected by “red-operculum” disease and corresponding water and sediment microbiota in four fish farm ponds. Distinct gut bacterial communities were observed in healthy and diseased fish. The bacterial communities of diseased fish were less diverse and stable than those of healthy individuals. The differences in bacterial community compositions between diseased and healthy fish were explained by the changes in the relative abundances of some specific bacterial OTUs, which belonged to the genera such as Vibrio, Aeromonas, and Shewanella, and they were prevalent in diseased fish, but rare or even absent in environmental samples. Water temperature and ammonia concentration were the two most important environmental factors that impacted gut microbiota in diseased fish. These results highlighted the surge of some potential pathogens as bacterial signatures that were associated with “red-operculum” disease in crucian carps.

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Acknowledgments

This work was supported by Sichuan Province Science and Technology Project (2017SZ0004, 2017JY0231), Tongwei Co., Ltd., China, and China Biodiversity Observation Networks (Sino BON).

Author’s Contributions

Xiangzhen Li, Tongtong Li, and Huan Li conceived the research. Tongtong Li, Huan Li, and Xuefeng Yan performed the experiments. Tongtong Li wrote the manuscript. Tongtong Li, Xiangzhen Li, Huan Li, and François-Joël Gatesoupe edited the manuscript. She Rong, Qiang Lin, Xuefeng Yan, and Jiabao Li contributed sampling, reagents, or data analysis pipeline. All authors reviewed and accepted the manuscript.

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Correspondence to Xiangzhen Li.

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Compliance with the ethics committee of the Chengdu Institute of Biology, Chinese Academy of Sciences, and the methods used in this study were carried out in accordance with the approved guidelines.

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The authors declare that they have no conflicts of interest.

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Li, T., Li, H., Gatesoupe, F. et al. Bacterial Signatures of “Red-Operculum” Disease in the Gut of Crucian Carp (Carassius auratus). Microb Ecol 74, 510–521 (2017). https://doi.org/10.1007/s00248-017-0967-1

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Keywords

  • Gut microbiota
  • Crucian carp
  • “Red-operculum” disease
  • Water temperature
  • Ammonia concentration