Microbial Ecology

, Volume 72, Issue 4, pp 955–964 | Cite as

High Nutrient Concentration Can Induce Virulence Factor Expression and Cause Higher Virulence in an Environmentally Transmitted Pathogen

  • Reetta Penttinen
  • Hanna Kinnula
  • Anssi Lipponen
  • Jaana K. H. Bamford
  • Lotta-Riina Sundberg
Host Microbe Interactions

Abstract

Environmentally transmitted opportunistic pathogens shuttle between two substantially different environments: outside-host and within-host habitats. These environments differ from each other especially with respect to nutrient availability. Consequently, the pathogens are required to regulate their behavior in response to environmental cues in order to survive, but how nutrients control the virulence in opportunistic pathogens is still poorly understood. In this study, we examined how nutrient level in the outside-host environment affects the gene expression of putative virulence factors of the opportunistic fish pathogen Flavobacterium columnare. The impact of environmental nutrient concentration on bacterial virulence was explored by cultivating the bacteria in various nutrient conditions, measuring the gene expression of putative virulence factors with RT-qPCR and, finally, experimentally challenging rainbow trout (Oncorhynchus mykiss) fry with these bacteria. Our results show that increased environmental nutrient concentration can increase the expression of putative virulence genes, chondroitinase (cslA) and collagenase, in the outside-host environment and may lead to more rapid fish mortality. These findings address that the environmental nutrients may act as significant triggers of virulence gene expression and therefore contribute to the interaction between an environmentally transmitted opportunistic pathogen and its host.

Keywords

Chondroitinase Collagenase Colony type Flavobacterium columnare Nutrients RT-qPCR Virulence factors 

Notes

Acknowledgments

The authors would like to thank Prof. Annemie Decostere for commenting on the manuscript and Ms Irene Helkala, Ville Hoikkala, MSc, Dr. Elina Laanto, Katja Neuvonen, MSc, Marjut Paljakka, MSc, Mr Petri Papponen, Dr. Katja Pulkkinen, and Dr. Ilona Rissanen, for assistance in the laboratory.

Compliance with Ethical Standards

The fish experiments were conducted according to the Finnish Act on Use of Animals for Experimental Purposes, under permission ESAVI-3940/04.10.07/2015 granted for L-RS by the National Animal Experiment Board at the Regional State Administrative Agency for Southern Finland.

Funding Information

This work was funded by Academy of Finland grant nos. 252411 (the Centre of Excellence in Biological Interactions 2012–2017) and 272995 (for L-RS), Maj and Tor Nessling Foundation, and the Doctoral Programme in Biological and Environmental Science (University of Jyväskylä). The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication in Microbial Ecology.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

248_2016_781_MOESM1_ESM.pdf (307 kb)
ESM 1 (PDF 306 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Reetta Penttinen
    • 1
  • Hanna Kinnula
    • 1
  • Anssi Lipponen
    • 2
  • Jaana K. H. Bamford
    • 1
  • Lotta-Riina Sundberg
    • 1
  1. 1.Department of Biological and Environmental Science and Nanoscience CenterUniversity of Jyvaskyla, Center of Excellence in Biological InteractionsUniversity of JyvaskylaFinland
  2. 2.University of Eastern Finland, A.I. Virtanen Institute for Molecular SciencesKuopioFinland

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