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Microbial Ecology

, Volume 72, Issue 1, pp 175–184 | Cite as

Methanogen Population of an Oil Production Skimmer Pit and the Effects of Environmental Factors and Substrate Availability on Methanogenesis and Corrosion Rates

  • Okoro Chuma Conlette
  • Nwezza Elebe Emmanuel
  • Okpokwasili Gideon Chijoke
Environmental Microbiology

Abstract

Assessment of microbial communities from an oil production skimmer pit using 16S rRNA gene sequencing technique revealed massive dominance of methanogenic archaea in both the skimmer pit water and sediment samples. The dominant genera of methanogens involved are mostly the acetotrophic Methanosaeta (36–83 %), and the hydrogenotrophic Methanococcus (49 %) indicating that methanogenesis is the dominant terminal metabolic process in the skimmer pit. Further studies showed that the methanogens had their optimal activity at pH 6–6.5, salinity of 100 mM, and temperature of 35–45 °C. When appropriate substrates are available and utilized by methanogens, methane production correlates with general corrosion rates (r = +0.927; p < 0.01), and under different conditions of pH, salinity and temperature, methane production showed significantly strong positive correlations (r = +0.824, +0.827, and +0.805; p < 0.01, respectively) with general corrosion rates. To the best of our knowledge, this research work was the first to assess microbial community composition of an oil production skimmer pit at Escravos facility in Nigeria.

Keywords

Skimmer pit Acetotrophic methanosaeta Hydrogenotrophic methanococcus Methanogenesis Environmental conditions 

Notes

Acknowledgments

This work was supported by grants from the Nigerian Petroleum Technology Development Fund (PTDF). We acknowledge the earlier DNA analysis and pyrosequencing carried out with grants from Gerrit Voordouw, University of Calgary, Alberta Canada.

Supplementary material

248_2016_764_MOESM1_ESM.docx (59 kb)
ESM 1 (DOCX 58 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Okoro Chuma Conlette
    • 1
  • Nwezza Elebe Emmanuel
    • 2
  • Okpokwasili Gideon Chijoke
    • 3
  1. 1.Department of Biology, Microbiology and BiotechnologyFederal UniversityIkwoNigeria
  2. 2.Department of Mathemetics/Computer science/Statistics/InformaticsFederal UniversityIkwoNigeria
  3. 3.Department of MicrobiologyUniversity of Port HarcourtPort HarcourtNigeria

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