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Microbial Ecology

, Volume 71, Issue 2, pp 339–346 | Cite as

ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS

  • Çağrı ErginEmail author
  • Yaşar Gök
  • Yasemin Bayğu
  • Ramazan Gümral
  • Betil Özhak-Baysan
  • Aylin Döğen
  • Dilara Öğünç
  • Macit Ilkit
  • Seyedmojtaba Seyedmousavi
Fungal Microbiology

Abstract

The present study compared two chemical-based methods, namely, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, to understand the misidentification of Exophiala dermatitidis and Exophiala phaeomuriformis. The study utilized 44 E. dermatitidis and 26 E. phaeomuriformis strains, which were partially treated with strong acids and bases for further evaluation. MALDI-TOF MS and ATR-FTIR spectroscopy data of the two Exophiala species were compared. Data groupings were observed for the chromic acid- and nitric acid-treated species when the black yeast sources were categorized as creosoted-oak sleepers, concrete sleepers, or dishwasher isolates. The MALDI-TOF MS data for the metalloenzyme-containing regions were consistent with the ATR-FTIR spectroscopy data. These results indicated that environmental isolates might contain metals not found in human isolates and might interfere with chemical-based identification methods. Therefore, MALDI-TOF MS reference libraries should be created for clinical strains and should exclude petroleum-associated environmental isolates.

Keywords

Chemical analysis İdentification İnfrared spectroscopy MALDI mass spectrometry Taxonomy 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Çağrı Ergin
    • 1
    Email author
  • Yaşar Gök
    • 2
  • Yasemin Bayğu
    • 2
  • Ramazan Gümral
    • 3
  • Betil Özhak-Baysan
    • 4
  • Aylin Döğen
    • 5
  • Dilara Öğünç
    • 4
  • Macit Ilkit
    • 6
  • Seyedmojtaba Seyedmousavi
    • 7
    • 8
    • 9
  1. 1.Department of Microbiology, Faculty of MedicinePamukkale UniversityDenizliTurkey
  2. 2.Chemistry Department, Faculty of Science and ArtsPamukkale UniversityDenizliTurkey
  3. 3.Department of MicrobiologyGülhane Military Medical AcademyAnkaraTurkey
  4. 4.Department of Microbiology, Faculty of MedicineUniversity of AkdenizAntalyaTurkey
  5. 5.Department of Pharmaceutical Microbiology, Faculty of PharmacyUniversity of MersinMersinTurkey
  6. 6.Division of Mycology, Department of Microbiology, Faculty of MedicineUniversity of ÇukurovaAdanaTurkey
  7. 7.Department of Medical Microbiology and Infectious DiseasesErasmus University Medical CenterRotterdamThe Netherlands
  8. 8.Department of Medical MicrobiologyRadboud University Medical CenterNijmegenThe Netherlands
  9. 9.Invasive Fungi Research CenterMazandaran University Medical CenterSariIran

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