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Microbial Ecology

, Volume 71, Issue 2, pp 505–517 | Cite as

A House for Two—Double Bacterial Infection in Euplotes woodruffi Sq1 (Ciliophora, Euplotia) Sampled in Southeastern Brazil

  • Marcus V. X. Senra
  • Roberto J. P. Dias
  • Michele Castelli
  • Inácio D. Silva-Neto
  • Franco Verni
  • Carlos A. G. SoaresEmail author
  • Giulio PetroniEmail author
Host Microbe Interactions

Abstract

Several ciliated protists form symbiotic associations with a diversity of microorganisms, leading to drastic impact on their ecology and evolution. In this work, two Euplotes spp. sampled in Rio de Janeiro, Brazil, were identified based on morphological and molecular features as Euplotes woodruffi strain Sq1 and E. encysticus strain Sq2 and investigated for the presence of endosymbionts. While E. woodruffi Sq1 stably hosts two bacterial populations, namely Polynucleobacter necessarius (Betaproteobacteria) and a new member of the family “Candidatus Midichloriaceae” (Alphaproteobacteria, Rickettsiales), here described as “Candidatus Bandiella woodruffii,” branching with a broad host range bacterial group found in association with cnidarians, sponges, euglenoids, and some arthropods; in E. encysticus Sq2 no symbiotic bacterium could be detected. The dispersion ability of this novel bacterium was tested by co-incubating E. woodruffi Sq1 with three different ciliate species. Among the tested strains “Ca. B. woodruffii” could only be detected in association with E. encysticus Sq2 with a prevalence of 20 % after 1 week and 40 % after 2 weeks, maintaining this level for up to 6 months. Nevertheless, this apparent in vitro association was abolished when E. woodruffi Sq1 donor was removed from the microcosm, suggesting that this bacterium has the capacity for at least a short-term survival outside its natural host and the aptitude to ephemerally interact with other organisms. Together, these findings strongly suggest the need for more detailed investigations to evaluate the host range for “Ca. B. woodruffii” and any possible pathogenic effect of this bacterium on other organisms including humans.

Keywords

Ciliates Bacterial symbionts Molecular phylogeny Symbiont dispersion 

Notes

Acknowledgements

The authors thank for their financial support the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (projects 562366/2010-5 and 484005/2013-8), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/110.022/2011), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); the Marie Curie Actions, European Commission FP7-PEOPLE-2009-IRSES project CINAR PATHOBACTER (project 247658) and the PRIN fellowship (protocol 2012A4F828_002) from the Italian Research Ministry (MIUR). During the period of development of this work, MS was also supported with Post-Doctoral fellowship by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), PNDP Institucional (project 006995/2011-51). S. Gabrielli is gratefully acknowledged for technical assistance in graphic artwork.

Supplementary material

248_2015_668_MOESM1_ESM.zip (340 kb)
ESM 1 Table S1-S3 Inventory of all sequences used on the phylogenetic analyses. Datasets: (S1) Euplotes; (S2) Polynucleobacter; and (S3) “Ca. Midichloriaceae”. (ZIP 339 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Marcus V. X. Senra
    • 1
    • 3
  • Roberto J. P. Dias
    • 2
    • 3
  • Michele Castelli
    • 4
  • Inácio D. Silva-Neto
    • 2
  • Franco Verni
    • 4
  • Carlos A. G. Soares
    • 1
    Email author
  • Giulio Petroni
    • 4
    Email author
  1. 1.Departamento de GenéticaUniversidade Federal do Rio de Janeiro, UFRJRio de JaneiroBrazil
  2. 2.Departamento de ZoologiaUniversidade Federal do Rio de Janeiro, UFRJRio de JaneiroBrazil
  3. 3.Departamento de ZoologiaUniversidade Federal de Juiz de Fora, UFJFRio de JaneiroBrazil
  4. 4.Department of BiologyUniversity of PisaPisaItaly

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