Microbial Ecology

, Volume 70, Issue 4, pp 948–960 | Cite as

Effect of the Organic Loading Rate Increase and the Presence of Zeolite on Microbial Community Composition and Process Stability During Anaerobic Digestion of Chicken Wastes

  • Elvira E. Ziganshina
  • Dmitry E. Belostotskiy
  • Olga N. Ilinskaya
  • Eugenia A. Boulygina
  • Tatiana V. Grigoryeva
  • Ayrat M. Ziganshin
Environmental Microbiology


This study investigates the effect of the organic loading rate (OLR) increase from 1.0 to 3.5 g VS L−1 day−1 at constant hydraulic retention time (HRT) of 35 days on anaerobic reactors’ performance and microbial diversity during mesophilic anaerobic digestion of ammonium-rich chicken wastes in the absence/presence of zeolite. The effects of anaerobic process parameters on microbial community structure and dynamics were evaluated using a 16S ribosomal RNA gene-based pyrosequencing approach. Maximum 12 % of the total ammonia nitrogen (TAN) was efficiently removed by zeolite in the fixed zeolite reactor (day 87). In addition, volatile fatty acids (VFA) in the fixed zeolite reactor accumulated in lower concentrations at high OLR of 3.2–3.5 g VS L−1 day−1. Microbial communities in the fixed zeolite reactor and reactor without zeolite were dominated by various members of Bacteroidales and Methanobacterium sp. at moderate TAN and VFA levels. The increase of the OLR accompanied by TAN and VFA accumulation and increase in pH led to the predominance of representatives of the family Erysipelotrichaceae and genera Clostridium and Methanosarcina. Methanosarcina sp. reached relative abundances of 94 and 57 % in the fixed zeolite reactor and reactor without zeolite at the end of the experimental period, respectively. In addition, the diminution of Synergistaceae and Crenarchaeota and increase in the abundance of Acholeplasmataceae in parallel with the increase of TAN, VFA, and pH values were observed.


Biogas Organic loading rate Zeolite Microbial diversity Ammonia inhibition Pyrosequencing 



The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University. Financial support was also provided by the Russian Foundation for Basic Research, Grant No. 14-08-31768. 454 Pyrosequencing was performed in the Interdisciplinary Center for Collective Use of Kazan Federal University for cellular, genomic, and post-genomic research in Volga Region (Russia).

Conflict of Interest

The authors declare that they have no competing interests.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Elvira E. Ziganshina
    • 1
  • Dmitry E. Belostotskiy
    • 2
  • Olga N. Ilinskaya
    • 1
  • Eugenia A. Boulygina
    • 3
  • Tatiana V. Grigoryeva
    • 3
  • Ayrat M. Ziganshin
    • 1
  1. 1.Department of MicrobiologyKazan (Volga Region) Federal UniversityKazanRussia
  2. 2.Department of Technologies, A. E. Arbuzov Institute of Organic and Physical ChemistryRussian Academy of SciencesKazanRussia
  3. 3.Laboratory of Omics TechnologiesKazan (Volga Region) Federal UniversityKazanRussia

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