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Microbial Ecology

, Volume 69, Issue 4, pp 895–904 | Cite as

Metagenomic Assessment of the Potential Microbial Nitrogen Pathways in the Rhizosphere of a Mediterranean Forest After a Wildfire

  • José F. Cobo-Díaz
  • Antonio J. Fernández-González
  • Pablo J. Villadas
  • Ana B. Robles
  • Nicolás Toro
  • Manuel Fernández-LópezEmail author
Environmental Microbiology

Abstract

Wildfires are frequent in the forests of the Mediterranean Basin and have greatly influenced this ecosystem. Changes to the physical and chemical properties of the soil, due to fire and post-fire conditions, result in alterations of both the bacterial communities and the nitrogen cycle. We explored the effects of a holm oak forest wildfire on the rhizospheric bacterial communities involved in the nitrogen cycle. Metagenomic data of the genes involved in the nitrogen cycle showed that both the undisturbed and burned rhizospheres had a conservative nitrogen cycle with a larger number of sequences related to the nitrogen incorporation pathways and a lower number for nitrogen output. However, the burned rhizosphere showed a statistically significant increase in the number of sequences for nitrogen incorporation (allantoin utilization and nitrogen fixation) and a significantly lower number of sequences for denitrification and dissimilatory nitrite reductase subsystems, possibly in order to compensate for nitrogen loss from the soil after burning. The genetic potential for nitrogen incorporation into the ecosystem was assessed through the diversity of the nitrogenase reductase enzyme, which is encoded by the nifH gene. We found that nifH gene diversity and richness were lower in burned than in undisturbed rhizospheric soils. The structure of the bacterial communities involved in the nitrogen cycle showed a statistically significant increase of Actinobacteria and Firmicutes phyla after the wildfire. Both approaches showed the important role of gram-positive bacteria in the ecosystem after a wildfire.

Keywords

Metagenomics Microbial communities Nitrogen cycle Rhizosphere Wildfire Mediterranean forest 

Notes

Acknowledgments

We would like to thank the authorities of the Sierra Nevada National Park for the access, facilities, and soil sampling. This work was funded by the following grants: P08-CVI-03549 from the Consejería de Innovación, Ciencia y Empresa of the Junta de Andalucía, OAPN 021/2007 and OAPN 748/2012 from the Organismo Autónomo Parques Nacionales (Ministry of the Environment), including ERDF (European Regional Development Fund). JFCD was awarded a predoctoral fellowship from the Junta de Andalucia, and AJFG was awarded a predoctoral fellowship (FPU) from the Spanish Ministry of Education.

Supplementary material

248_2015_586_MOESM1_ESM.docx (2.6 mb)
Fig. S1 Description of the sampled areas. A) Location of study areas with its GPS position coordinates, altitude and kind of vegetation. B) Geographic location of the sampled sites at Sierra Nevada. UOF, undisturbed holm-oak forest; BOF, burned holm-oak forest; BBS, burned bulk soil with grasses and shrub before the wildfire. (DOCX 2696 kb)
248_2015_586_MOESM2_ESM.docx (102 kb)
Fig. S2 Rarefaction curves (A) and coverage table (B) for the nifH gene clone libraries. UOF, undisturbed holm-oak forest; BOF, burned holm-oak forest; BBS, burned bulk soil with grasses and shrub before the wildfire. (DOCX 102 kb)
248_2015_586_MOESM3_ESM.docx (86 kb)
Fig. S3 Agglomerative hierarchical clustering of the NifH proteins of each library with the Euclidean distance matrix and UPGMA algorithm. Analyses were carried out on the abundance of each OTU defined by a 93 % similarity cut-off. The name of each branch corresponds to the sampled site: UOF (undisturbed holm-oak forest), BOF (rhizosphere of burned holm-oak forest), BBS (burned bulk soil). (DOCX 86 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • José F. Cobo-Díaz
    • 1
  • Antonio J. Fernández-González
    • 1
  • Pablo J. Villadas
    • 1
  • Ana B. Robles
    • 2
  • Nicolás Toro
    • 1
  • Manuel Fernández-López
    • 1
    Email author
  1. 1.Grupo de Ecología Genética de la Rizosfera, Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del ZaidínConsejo Superior de Investigaciones CientíficasGranadaSpain
  2. 2.Grupo de Pastos y Sistemas Silvopastorales Mediterráneos, Estación Experimental del ZaidínConsejo Superior de Investigaciones CientíficasGranadaSpain

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