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Microbial Ecology

, Volume 70, Issue 2, pp 545–556 | Cite as

The Gut Microbiota of Workers of the Litter-Feeding Termite Syntermes wheeleri (Termitidae: Syntermitinae): Archaeal, Bacterial, and Fungal Communities

  • Renata Henrique Santana
  • Elisa Caldeira Pires Catão
  • Fabyano Alvares Cardoso Lopes
  • Reginaldo Constantino
  • Cristine Chaves Barreto
  • Ricardo Henrique KrügerEmail author
Host Microbe Interactions

Abstract

The gut microbiota of termites allows them to thrive on a variety of different materials such as wood, litter, and soil. For that reason, they play important roles in the decomposition of biomass in diverse biomes. This function is essential in the savanna, where litter-feeding termites are one of the few invertebrates active during the dry season. In this study, we describe the gut microbiota of workers (third and fourth instars) of the species Syntermes wheeleri, a litter-feeding termite from the Brazilian savanna. Results of 16S and 18S ribosomal RNA (rRNA) gene-targeted pyrosequencing using primers sets specific to each domain have revealed its bacterial, archaeal, and fungal diversities. Firmicutes accounted for more than half of the operational taxonomic units of the Bacteria domain. The most abundant fungal species were from the class Dothideomycetes of the phylum Ascomycota. The methanogenic orders Methanobacteriales, Methanosarcinales, and Methanomicrobiales of the phylum Euryarchaeota accounted for the greatest part of the Archaea detected in this termite. A comparison of the gut microbiota of the two instars revealed a difference in operational taxonomic unit (OTU) abundance but not in species richness. This description of the whole gut microbiota represents the first study to evaluate relationships among bacteria, archaea, fungi, and host in S. wheeleri.

Keywords

Termite Gut microbiota Brazilian savanna Pyrosequencing 16S rRNA gene 18S rRNA gene 

Notes

Acknowledgments

This work was supported by grants from the Federal District Research Support Foundation (FAPDF) and National Council for Scientific and Technological Development (CNPq). We thank Dr. Andreas Brune for sharing the DictDb v. 2.3 database.

Supplementary material

248_2015_581_Fig4_ESM.gif (27 kb)
Figure S1

Relative abundance of OTUs from Firmicutes phylum at family level. (GIF 26 kb)

248_2015_581_MOESM1_ESM.tif (444 kb)
High resolution image (TIFF 444 kb)
248_2015_581_Fig5_ESM.gif (54 kb)
Figure .S2

Abundance of microorganisms differed significantly between the third- and fourth-instar workers of Syntermes wheeleri: (a) the bacterial phylum TG3, (b) the archaeal order Methanobacteriales; and (c) fungal classes. STAMP analysis using two-sided Fisher’s exact test with Bonferroni multiple test correction and effect size filter (ratio of proportions < 2.0). (GIF 53 kb)

248_2015_581_MOESM2_ESM.tif (322 kb)
High resolution image (TIFF 321 kb)
248_2015_581_MOESM3_ESM.xlsx (34 kb)
ESM 1 (XLSX 34 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Renata Henrique Santana
    • 1
  • Elisa Caldeira Pires Catão
    • 2
  • Fabyano Alvares Cardoso Lopes
    • 2
  • Reginaldo Constantino
    • 3
  • Cristine Chaves Barreto
    • 1
  • Ricardo Henrique Krüger
    • 2
    • 4
    Email author
  1. 1.Genomic Sciences and BiotechnologyUniversidade Católica de BrasíliaBrasíliaBrazil
  2. 2.Cellular Biology DepartmentUniversidade de BrasíliaBrasíliaBrazil
  3. 3.Zoology DepartmentUniversidade de BrasíliaBrasíliaBrazil
  4. 4.Departamento de Biologia Celular, Instituto Central de Ciências Sul, Laboratório de EnzimologiaUniversidade de Brasília—UnBBrasíliaBrazil

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