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Microbial Ecology

, Volume 69, Issue 2, pp 422–433 | Cite as

Stability and Resilience of Oral Microcosms Toward Acidification and Candida Outgrowth by Arginine Supplementation

  • Jessica E. KoopmanEmail author
  • Wilfred F. M. Röling
  • Mark J. Buijs
  • Christopher H. Sissons
  • Jacob M. ten Cate
  • Bart J. F. Keijser
  • Wim Crielaard
  • Egija Zaura
Host Microbe Interactions

Abstract

Dysbiosis induced by low pH in the oral ecosystem can lead to caries, a prevalent bacterial disease in humans. The amino acid arginine is one of the pH-elevating agents in the oral cavity. To obtain insights into the effect of arginine on oral microbial ecology, a multi-plaque “artificial mouth” (MAM) biofilm model was inoculated with saliva from a healthy volunteer and microcosms were grown for 4 weeks with 1.6 % (w/v) arginine supplement (Arginine) or without (Control), samples were taken at several time-points. A cariogenic environment was mimicked by sucrose pulsing. The bacterial composition was determined by 16S rRNA gene amplicon sequencing, the presence and amount of Candida and arginine deiminase system genes arcA and sagP by qPCR. Additionally, ammonium and short-chain fatty acid concentrations were determined. The Arginine microcosms were dominated by Streptococcus, Veillonella, and Neisseria and remained stable in time, while the composition of the Control microcosms diverged significantly in time, partially due to the presence of Megasphaera. The percentage of Candida increased 100-fold in the Control microcosms compared to the Arginine microcosms. The pH-raising effect of arginine was confirmed by the pH and ammonium results. The abundances of sagP and arcA were highest in the Arginine microcosms, while the concentration of butyrate was higher in the Control microcosms. We demonstrate that supplementation with arginine serves a health-promoting function; it enhances microcosm resilience toward acidification and suppresses outgrowth of the opportunistic pathogen Candida. Arginine facilitates stability of oral microbial communities and prevents them from becoming cariogenic.

Keywords

Arginine Candida Oral Ecology Microcosm 

Notes

Acknowledgments

We are grateful to Dr. Lisa Wong for her advice on operating MAM. We like to thank Dr. Bernd Brandt for his advice on bioinformatics, Elly van Deutekom-Mulder, Joyce van der Horst, and Marta ter Haar for technical support. We are very grateful to our saliva donor.

Supplementary material

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Table S1 (PDF 84 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jessica E. Koopman
    • 1
    Email author
  • Wilfred F. M. Röling
    • 2
  • Mark J. Buijs
    • 1
  • Christopher H. Sissons
    • 3
  • Jacob M. ten Cate
    • 1
  • Bart J. F. Keijser
    • 4
  • Wim Crielaard
    • 1
  • Egija Zaura
    • 1
  1. 1.Department of Preventive Dentistry, Academic Centre for Dentistry AmsterdamUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Department of Molecular Cell PhysiologyVU University AmsterdamAmsterdamNetherlands
  3. 3.Department of Pathology and Molecular MedicineUniversity of OtagoWellingtonNew Zealand
  4. 4.Microbiology and Systems BiologyTNO Earth, Environmental and Life SciencesZeistThe Netherlands

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