Identification of mVOCs from Andean Rhizobacteria and Field Evaluation of Bacterial and Mycorrhizal Inoculants on Growth of Potato in its Center of Origin
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Food security (a pressing issue for all nations) faces a threat due to population growth, land availability for growing crops, a changing climate (leading to increases in both abiotic and biotic stresses), heightened consumer awareness of the risks related to the use of agrichemicals, and also the reliance on depleting fossil fuel reserves for their production. Legislative changes in Europe mean that fewer agrichemicals will be available in the future for the control of crop pests and pathogens. The need for the implementation of a more sustainable agricultural system globally, incorporating an integrated approach to disease management, has never been more urgent. To that end, the Valorizing Andean Microbial Diversity (VALORAM) project (http://valoram.ucc.ie), funded under FP7, examined the role of microbial communities in crop production and protection to improve the sustainability, food security, environmental protection, and productivity for rural Andean farmers. During this work, microbial volatile organic compounds (mVOCs) of 27 rhizobacterial isolates were identified using gas chromatography/mass spectrometry (GC/MS), and their antifungal activity against Rhizoctonia solani was determined in vitro and compared to the activity of a selection of pure volatile compounds. Five of these isolates, Pseudomonas palleroniana R43631, Bacillus sp. R47065, R47131, Paenibacillus sp. B3a R49541, and Bacillus simplex M3-4 R49538 trialled in the field in their respective countries of origin, i.e., Bolivia, Peru, and Ecuador, showed significant increase in the yield of potato. The strategy followed in the VALORAM project may offer a template for the future isolation and determination of putative biocontrol and plant growth-promoting agents, useful as part of a low-input integrated pest management system.
KeywordsmVOCs Sustainability Andean potato Rhizobacteria AMF Biocontrol
The research project “VALORAM-Valorizing Andean microbial diversity through sustainable intensification of potato-based farming systems” was supported by European Commission’s Seventh Framework Program FP7/2007-2013 under grant agreement No 227522, 01/02/2009-31/01/2014.
- 11.Velázquez-Becerra C, Macías-Rodríguez L, López-Bucio J, Altamirano-Hernández J, Flores-Cortez I, Valencia-Cantero E (2011) A volatile organic compound analysis from Arthrobacter agilis identifies dimethylhexadecylamine, an amino-containing lipid modulating bacterial growth and Medicago sativa morphogenesis in vitro. Plant Soil 339:329–340. doi: 10.1007/s11104-010-0583-z CrossRefGoogle Scholar
- 13.Blom D, Fabbri C, Connor EC, Schiestl FP, Klauser DR, Boller T, Eberl L, Weisskopf L (2011) Production of plant growth modulating volatiles is widespread among rhizosphere bacteria and strongly depends on culture conditions. Environ Microbiol 13:3047–3058. doi: 10.1111/j.1462-2920.2011.02582.x CrossRefPubMedGoogle Scholar
- 21.Ghyselinck J, Velivelli SL, Heylen K, O’Herlihy E, Franco J, Rojas M, De Vos P, Prestwich BD (2013) Bioprospecting in potato fields in the Central Andean Highlands: screening of rhizobacteria for plant growth-promoting properties. Syst Appl Microbiol 36:116–127. doi: 10.1016/j.syapm.2012.11.007 CrossRefPubMedGoogle Scholar
- 22.Velivelli S, O’Herlihy E, Janczura B, Doyle Prestwich B, Ghyselinck J, De Vos P (2012) Efficacy of rhizobacteria on plant growth-promotion and disease suppression in vitro. Acta Horticult 961:525–532Google Scholar
- 23.Athukorala SNP, Fernando WGD, Rashid KY, de Kievit T (2010) The role of volatile and non-volatile antibiotics produced by Pseudomonas chlororaphis strain PA23 in its root colonization and control of Sclerotinia sclerotiorum. Biocontrol Sci Tech 20:875–890. doi: 10.1080/09583157.2010.484484 CrossRefGoogle Scholar
- 29.Dandurishvili N, Toklikishvili N, Ovadis M, Eliashvili P, Giorgobiani N, Keshelava R, Tediashvili M, Vainstein A, Khmel I, Szegedi E, Chernin L (2011) Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants. J Appl Microbiol 110:341–352. doi: 10.1111/j.1365-2672.2010.04891.x CrossRefPubMedGoogle Scholar
- 33.Weise T, Kai M, Gummesson A, Troeger A, von Reuss S, Piepenborn S, Kosterka F, Sklorz M, Zimmermann R, Francke W, Piechulla B (2012) Volatile organic compounds produced by the phytopathogenic bacterium Xanthomonas campestris pv. vesicatoria 85–10. Beilstein J Org Chem 8:579–596. doi: 10.3762/bjoc.8.65 CrossRefPubMedCentralPubMedGoogle Scholar
- 38.Spinelli F, Cellini A, Vanneste J, Rodriguez-Estrada M, Costa G, Savioli S, Harren FM, Cristescu S (2012) Emission of volatile compounds by Erwinia amylovora: biological activity in vitro and possible exploitation for bacterial identification. Trees 26:141–152. doi: 10.1007/s00468-011-0667-2 CrossRefGoogle Scholar
- 39.de Faria, S.M., Resende A.S., Júnior O.J.S., Boddey, R.M.: Exploiting mycorrhizae and Rhizobium symbioses to recover seriously gegraded soils. In: Polacco, JC, Todd, CD (eds.) Ecological Aspects of Nitrogen Metabolism in Plants. John Wiley & Sons, Inc., pp. 195–215. (2011)Google Scholar
- 40.Compant S, Duffy B, Nowak J, Clément C, Barka EA (2005) Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl Environ Microbiol 71:4951–4959. doi: 10.1128/aem. 71.9.4951-4959.2005 CrossRefPubMedCentralPubMedGoogle Scholar