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Microbial Ecology

, Volume 66, Issue 1, pp 96–104 | Cite as

Illumina Sequencing of 16S rRNA Tag Revealed Spatial Variations of Bacterial Communities in a Mangrove Wetland

  • Xiao-Tao Jiang
  • Xin Peng
  • Guan-Hua Deng
  • Hua-Fang Sheng
  • Yu Wang
  • Hong-Wei ZhouEmail author
  • Nora Fung-Yee TamEmail author
Environmental Microbiology

Abstract

The microbial community plays an essential role in the high productivity in mangrove wetlands. A proper understanding of the spatial variations of microbial communities will provide clues about the underline mechanisms that structure microbial groups and the isolation of bacterial strains of interest. In the present study, the diversity and composition of the bacterial community in sediments collected from four locations, namely mudflat, edge, bulk, and rhizosphere, within the Mai Po Ramsar Wetland in Hong Kong, SAR, China were compared using the barcoded Illumina paired-end sequencing technique. Rarefaction results showed that the bulk sediment inside the mature mangrove forest had the highest bacterial α-diversity, while the mudflat sediment without vegetation had the lowest. The comparison of β-diversity using principal component analysis and principal coordinate analysis with UniFrac metrics both showed that the spatial effects on bacterial communities were significant. All sediment samples could be clustered into two major groups, inner (bulk and rhizosphere sediments collected inside the mangrove forest) and outer mangrove sediments (the sediments collected at the mudflat and the edge of the mangrove forest). With the linear discriminate analysis scores larger than 3, four phyla, namely Actinobacteria, Acidobacteria, Nitrospirae, and Verrucomicrobia, were enriched in the nutrient-rich inner mangrove sediments, while abundances of Proteobacteria and Deferribacterias were higher in outer mangrove sediments. The rhizosphere effect of mangrove plants was also significant, which had a lower α-diversity, a higher amount of Nitrospirae, and a lower abundance of Proteobacteria than the bulk sediment nearby.

Keywords

Bacterial Community Proteobacteria Mangrove Forest Terminal Restriction Fragment Length Polymorphism Bulk Sediment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC 31270152), the Guangdong Natural Science Foundation (no. S2011010004136), the Program for New Century Excellent Talents in University (NCET-11-0921), and Educational Commission of Guangdong Province, China (2012KJCX0031). The authors would also like to acknowledge the financial support from the State Key Laboratory in Marine Pollution, City University of Hong Kong.

Supplementary material

248_2013_238_MOESM1_ESM.pdf (116 kb)
ESM 1 PDF 116 kb

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Xiao-Tao Jiang
    • 1
  • Xin Peng
    • 1
  • Guan-Hua Deng
    • 1
  • Hua-Fang Sheng
    • 1
  • Yu Wang
    • 1
  • Hong-Wei Zhou
    • 1
    Email author
  • Nora Fung-Yee Tam
    • 2
    • 3
    Email author
  1. 1.Department of Environmental Health, School of Public Health and Tropical MedicineSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Biology and ChemistryCity University of Hong KongHong KongChina
  3. 3.State Key Laboratory in Marine PollutionCity University of Hong KongHong KongChina

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