Microbial Ecology

, Volume 66, Issue 1, pp 158–170 | Cite as

Agricultural Management and Labile Carbon Additions Affect Soil Microbial Community Structure and Interact with Carbon and Nitrogen Cycling

  • Sean T. Berthrong
  • Daniel H. Buckley
  • Laurie E. Drinkwater
Soil Microbiology

Abstract

We investigated how conversion from conventional agriculture to organic management affected the structure and biogeochemical function of soil microbial communities. We hypothesized the following. (1) Changing agricultural management practices will alter soil microbial community structure driven by increasing microbial diversity in organic management. (2) Organically managed soil microbial communities will mineralize more N and will also mineralize more N in response to substrate addition than conventionally managed soil communities. (3) Microbial communities under organic management will be more efficient and respire less added C. Soils from organically and conventionally managed agroecosystems were incubated with and without glucose (13C) additions at constant soil moisture. We extracted soil genomic DNA before and after incubation for TRFLP community fingerprinting of soil bacteria and fungi. We measured soil C and N pools before and after incubation, and we tracked total C respired and N mineralized at several points during the incubation. Twenty years of organic management altered soil bacterial and fungal community structure compared to continuous conventional management with the bacterial differences caused primarily by a large increase in diversity. Organically managed soils mineralized twice as much NO3 as conventionally managed ones (44 vs. 23 μg N/g soil, respectively) and increased mineralization when labile C was added. There was no difference in respiration, but organically managed soils had larger pools of C suggesting greater efficiency in terms of respiration per unit soil C. These results indicate that the organic management induced a change in community composition resulting in a more diverse community with enhanced activity towards labile substrates and greater capacity to mineralize N.

Supplementary material

248_2013_225_MOESM1_ESM.pdf (25 kb)
Fig. S1Soil incubator design. Incubators were built with sterilized plastic vacuum filtration units with a 0.2-μm PES filter. A 45-μm glass fiber prefilter was secured with silicon sealant above the nylon filter to avoid clogging. Inert PTFE chips were added in the center of the incubator to support a 30-ml Erlenmeyer flask with 0.5 M KOH (the CO2 trap). During simulated rainfall events, the trap was removed and replaced, and between rainfalls, the incubators were sealed with a lid and parafilm (PDF 25 kb)
248_2013_225_MOESM2_ESM.pdf (24 kb)
Fig. S2Schematic diagram of the steps in the incubator process (PDF 24 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sean T. Berthrong
    • 1
  • Daniel H. Buckley
    • 2
  • Laurie E. Drinkwater
    • 1
  1. 1.Department of HorticultureCornell UniversityIthacaUSA
  2. 2.Department of Crop and Soil SciencesCornell UniversityIthacaUSA

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