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Microbial Ecology

, Volume 65, Issue 4, pp 1076–1086 | Cite as

Metagenomic Analysis of Healthy and White Plague-Affected Mussismilia braziliensis Corals

  • Gizele D. Garcia
  • Gustavo B. Gregoracci
  • Eidy de O. Santos
  • Pedro M. Meirelles
  • Genivaldo G. Z. Silva
  • Rob Edwards
  • Tomoo Sawabe
  • Kazuyoshi Gotoh
  • Shota Nakamura
  • Tetsuya Iida
  • Rodrigo L. de Moura
  • Fabiano L. Thompson
Host Microbe Interactions

Abstract

Coral health is under threat throughout the world due to regional and global stressors. White plague disease (WP) is one of the most important threats affecting the major reef builder of the Abrolhos Bank in Brazil, the endemic coral Mussismilia braziliensis. We performed a metagenomic analysis of healthy and WP-affected M. braziliensis in order to determine the types of microbes associated with this coral species. We also optimized a protocol for DNA extraction from coral tissues. Our taxonomic analysis revealed Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, and Actinomycetes as the main groups in all healthy and WP-affected corals. Vibrionales, members of the CytophagaFlavobacteriumBacteroides complex, Rickettsiales, and Neisseriales were more abundant in the WP-affected corals. Diseased corals also had more eukaryotic metagenomic sequences identified as Alveolata and Apicomplexa. Our results suggest that WP disease in M. braziliensis is caused by a polymicrobial consortium.

Keywords

Vibrio Bacteroidetes Coral Species Metagenomic Library Metagenomic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We acknowledge JST-CNPq, FAPERJ, and CAPES for funding. We also thank the project for the International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, from the Ministry of Education, Science, Sports, Culture, and Technology, Japan.

Supplementary material

248_2012_161_MOESM1_ESM.xlsx (11 kb)
Table 1 Coral Database composition and references (XLSX 11 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Gizele D. Garcia
    • 1
  • Gustavo B. Gregoracci
    • 1
  • Eidy de O. Santos
    • 1
  • Pedro M. Meirelles
    • 1
  • Genivaldo G. Z. Silva
    • 1
    • 2
  • Rob Edwards
    • 1
    • 2
  • Tomoo Sawabe
    • 3
  • Kazuyoshi Gotoh
    • 4
  • Shota Nakamura
    • 4
  • Tetsuya Iida
    • 4
  • Rodrigo L. de Moura
    • 1
  • Fabiano L. Thompson
    • 1
    • 5
  1. 1.Institute of BiologyFederal University of Rio de Janeiro (UFRJ)Rio de JaneiroBrazil
  2. 2.Computational Science Research CenterSan Diego State UniversitySan DiegoUSA
  3. 3.Laboratory of Microbiology, Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan
  4. 4.Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial DiseasesOsaka UniversityOsakaJapan
  5. 5.Rio de JaneiroBrazil

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