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Microbial Ecology

, Volume 65, Issue 2, pp 394–404 | Cite as

Elevated Atmospheric CO2 Impacts Abundance and Diversity of Nitrogen Cycling Functional Genes in Soil

  • John J. KellyEmail author
  • Emily Peterson
  • Jonathan Winkelman
  • Teagan J. Walter
  • Steven T. Rier
  • Nancy C. Tuchman
Soil Microbiology

Abstract

The concentration of CO2 in the Earth's atmosphere has increased over the last century. Although this increase is unlikely to have direct effects on soil microbial communities, increased atmospheric CO2 may impact soil ecosystems indirectly through plant responses. This study tested the hypothesis that exposure of plants to elevated CO2 would impact soil microorganisms responsible for key nitrogen cycling processes, specifically denitrification and nitrification. We grew trembling aspen (Populus tremuloides) trees in outdoor chambers under ambient (360 ppm) or elevated (720 ppm) levels of CO2 for 5 years and analyzed the microbial communities in the soils below the trees using quantitative polymerase chain reaction and clone library sequencing targeting the nitrite reductase (nirK) and ammonia monooxygenase (amoA) genes. We observed a more than twofold increase in copy numbers of nirK and a decrease in nirK diversity with CO2 enrichment, with an increased predominance of Bradyrhizobia-like nirK sequences. We suggest that this dramatic increase in nirK-containing bacteria may have contributed to the significant loss of soil N in the CO2-treated chambers. Elevated CO2 also resulted in a significant decrease in copy numbers of bacterial amoA, but no change in archaeal amoA copy numbers. The decrease in abundance of bacterial amoA was likely a result of the loss of soil N in the CO2-treated chambers, while the lack of response for archaeal amoA supports the hypothesis that physiological differences in these two groups of ammonia oxidizers may enable them to occupy distinct ecological niches and respond differently to environmental change.

Keywords

Clone Library amoA Gene ELEV Soil Bacterial amoA Gene nirK Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Soils analyzed in this project were collected at the Elevated CO2 Research Facility of the University of Michigan Biological Station, where infrastructural support was provided by the US DOE National Institute of Global Environmental Change (NIGEC). This research was supported, in part, by grants awarded to N.C.T. from the National Science Foundation (DEB-9903888 and DEB-0108847) and by a supplement to grant DEB-0108847 awarded to N.C.T. and J.J.K. from the National Science Foundation. T.W. was supported by the Loyola University Chicago NSF REU Program in Bioinformatics (DBI 0552888). E.P. was supported by a Loyola University Chicago Provost Fellowship. The authors thank Kesha Baxi and Sara Idleman for their contributions to clone library preparation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • John J. Kelly
    • 1
    Email author
  • Emily Peterson
    • 1
  • Jonathan Winkelman
    • 1
  • Teagan J. Walter
    • 2
  • Steven T. Rier
    • 3
  • Nancy C. Tuchman
    • 1
  1. 1.Department of BiologyLoyola University ChicagoChicagoUSA
  2. 2.Department of BiologyCarleton CollegeNorthfieldUSA
  3. 3.Department of Biological and Allied Health SciencesBloomsburg UniversityBloomsburgUSA

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