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Microbial Ecology

, Volume 65, Issue 1, pp 255–267 | Cite as

Revised Systematics of Holospora-Like Bacteria and Characterization of “Candidatus Gortzia infectiva”, a Novel Macronuclear Symbiont of Paramecium jenningsi

  • Vittorio Boscaro
  • Sergei I. Fokin
  • Martina Schrallhammer
  • Michael Schweikert
  • Giulio PetroniEmail author
Host Microbe Interactions

Abstract

The genus Holospora (Rickettsiales) includes highly infectious nuclear symbionts of the ciliate Paramecium with unique morphology and life cycle. To date, nine species have been described, but a molecular characterization is lacking for most of them. In this study, we have characterized a novel Holospora-like bacterium (HLB) living in the macronuclei of a Paramecium jenningsi population. This bacterium was morphologically and ultrastructurally investigated in detail, and its life cycle and infection capabilities were described. We also obtained its 16S rRNA gene sequence and developed a specific probe for fluorescence in situ hybridization experiments. A new taxon, “Candidatus Gortzia infectiva”, was established for this HLB according to its unique characteristics and the relatively low DNA sequence similarities shared with other bacteria. The phylogeny of the order Rickettsiales based on 16S rRNA gene sequences has been inferred, adding to the available data the sequence of the novel bacterium and those of two Holospora species (Holospora obtusa and Holospora undulata) characterized for the purpose. Our phylogenetic analysis provided molecular support for the monophyly of HLBs and showed a possible pattern of evolution for some of their features. We suggested to classify inside the family Holosporaceae only HLBs, excluding other more distantly related and phenotypically different Paramecium endosymbionts.

Keywords

Uncultured Bacterium Bacterial Symbiont Restriction Fragment Length Polymorphism Pattern Magnetospirillum Paramecium Bursaria 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Ms. T. Fokina for the lucky sampling in Thailand, Prof. E. Przyboś for the P. quadecaurelia identification, Prof. O. Kaltz for providing the P. caudatum cultures and the anonymous reviewers who gave very useful advices, improving the quality of this paper. S. Gabrielli is gratefully acknowledged for technical assistance in graphic artwork. This work was supported by PRIN fellowship (protocol 2008TRZSXF_002) from the Italian Research Ministry (MIUR), the Volkswagen foundation (project number: 84816), the European Commission FP7-PEOPLE-2009-IRSES project CINAR PATHOBACTER (247658) and the support actions for international academic cooperation of Pisa University (years 2011/2012).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Vittorio Boscaro
    • 1
  • Sergei I. Fokin
    • 1
  • Martina Schrallhammer
    • 2
  • Michael Schweikert
    • 3
  • Giulio Petroni
    • 1
    Email author
  1. 1.Biology Department, Protistology-Zoology UnitUniversity of PisaPisaItaly
  2. 2.Institute of HydrobiologyDresden University of TechnologyDresdenGermany
  3. 3.Biological InstituteStuttgart UniversityStuttgartGermany

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